ムラサキイガイ前足糸牽引筋の筋小胞体内Ca 結合蛋白質に関する免疫電子顕微鏡的研究

概要

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To examine whether the sarcoplasmic reticulum (SR) of Mytilus anterior byssus retractor muscle (ABRM) contains calsequestrin as a Ca-binding protein, immuno-electron microscope observations were performed by using the anti-calsequestrin antibody. For the immuno-electron microscope study, the ABRM fibers were chemically fixed with a paraformaldehyde solution, dehydrated and embedded in Lowicryl K4M resin. Ultrathin sections were treated successively with anti-calsequestrin IgG and goat anti-rabbit IgG conjugated with colloid gold. Then they were examined with a JEM 2000EX electron microscope. In the ABRM fibers prepared with the conventional fixation procedure, the SR located along the inner surface of plasma membrane contained many clusters of numerous electron-dense particles in its lumen and on the inner surface of SR membrane. When the sections were stained with antibodies, the gold particles recognizing the existence of calsequestrin were exclusively found on the clusters of electron-dense particles in the SR. This result indicates that the major Ca-binding protein of SR is calsequestrin in the ABRM fibers, and is located not only in the SR lumen but also on the inner surface of SR membrane.
2010-06-30