[原著］Genetic influence of HLA-DR on longevity in Okinawan people

概要

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Okinawa is well-known as one of the longevity areas in the world and the population rate of centenarians is higher by far than that of the whole of Japan. However, only few reports have used genetic approaches to explain Okinawan long lifespan. In this study, we analyzed human luekocyte antigen (HLA) both in phenotyping and in genotyping for the same subjects of Okinawan centenarians and normal adults for the purpose of clarifying one of the primary genetic factors in major histo-compatibility complex (MHC) region genes associated with longevity. Eighty-six healthy centenarians and 142 normal adults in Okinawa were studied. For HLA phenotyping, 14 antigen specificities on DR locus were typed according to the standard microdroplet lymphocyte cytotoxicity test, and for genotyping 27 alleles on DRBl were typed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method based on the digestion of PCR-amplified DNAs with allele specific enzymes. In the present study, DR1 was found to be high in centenarians in phenotyping (P=0.029, RR=5.779), which was similar in genotypeing (DRB1^*0101: P=0.030, RR=5.779), but both results were not significantly different at Pc level. However, DRB1^*1401 (P=0.00103, Pc=0.028, RR-3.456) was significantly high. These data suggest that several alleles of HLA-DRB1, such as DRB1^*1401, favourably relate to longevity.
琉球医学会の論文