[原著］Expression of interleukin-7 in the model of orthotopic liver transplantation in rats
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概要
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The purpose of this experiment was to determine whether interleukin-7 (IL-7) is involved in the development of acute liver rejection in the model of orthotopic liver transplantation in rats. We evaluated IL-7 expression using samples serially obtained from the various organs, on 1, 3, 5, 7, 10 post-operative day (POD, n=5 at each POD) in two groups: Group I --BN (RT1^n) to BN rats; Group II --DA (RTl^a) to BN rats. IL-7 gene sequence and IL-7 mRNA were detected with PCR and Northern blotting. IL-7 levels were determined with enzyme-linked immunosorbent assay (ELISA), and data were expressed as $mean \pm standard$ error. Group I animals (n=5) survived indefinitely. Whereas group II animals (n=5) died within $12.8 \pm 2.7$ days due to the acute rejection proved histologically. In both groups, IL-7 gene sequence could be detected in every organ examined by PCR. In Northern blotting, IL-7 mRNA could be detected in lOO% of thymic and bone marrow samples, and one third ofthekidney in both groups. In ELISA, IL-7 levels of group I remained low throughout the experiment in each organ, whereas in group II, IL-7 levels increased until POD 10, with statisticaly significant differences against those in group I on POD 10, in the serum, liver and thymus (p<0.05) (serum--$3.60 \pm 0.39$ vs. $0.63 \pm 0.09$, liver--$7.29 \pm 1.4$ vs. $2.66 \pm 0.38$, thymus--$13.3 \pm 5.8$ vs. $0.91 \pm 0.32$). In conclusion, IL-7 was increasingly produced in the thymus during the acute rejection of the graft liver. The thymus and its IL-7 are thought to be playing an important role in the acute rejection of the graft liver.
- 琉球医学会の論文
- 1997-00-00
琉球医学会 | 論文
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- [原著］Expression of interleukin-7 in the model of orthotopic liver transplantation in rats
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