［原著］Mechanical Stress regulates Chondrocyte Proliferation and Differentiation during Endochondral Bone Formation
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概要
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The effects of mechanical stress on the proliferation and differentiation of mesenchymal progenitor cells toward chondrogenesis are not well addressed. The purpose of this study was to assess how mechanical stress exerts the influence on chondrogenic proliferation and differentiation during endochondral bone formation. ATDC5 mouse chondroprogenitor cells, which undergo a reproducible multistep chondrogenic differentiation in response to insulin, were used in this study. Mechanical loading was applied to ATDC5 cells grown on plastic plates in monolayer cultures at a magnitude of 2800 $ \mu $strain and a frequency of 0.5 Hz for 24 hr at day 4, 7, 14, 21 and 28. The chondrogenesis pathway m ATDC5 cells was studied by analyzing differentiation stage-specific gene expression using a Northern blot analysis. ATDC5 cells responded to the addition of insulin with a significant increase in the expression of mRNA for histone H4, type II collagen, and aggrecan, but not type X collagen during a 28-day culture period. These inductive responses were time-dependent, and therefore, differentiation-dependent. Applying mechamcal loading in the presence of insulin further up-regulated the mRNA levels of these proteins including type X collagen. Moreover, mechanical loading increased the histone H4 mRNA expression in immature cells, and promoted the mRNA expression of cartilage-type extracellular matrix in insulin-induced relatively mature cells, which also indicated differentiation-dependent. The expression of histone H4, type II and X collagen, and aggrecan mRNA levels were also increased with mechanical loading alone. These results demonstrated the adjunctive effects of mechanical loading on insulininduced chondrogenesis and the peculiar effects of mechanical loading on the induction of chondrogenesis, thus suggesting that mechanical loading can promote endochondral bone formation.
- 琉球医学会の論文
- 2007-00-00
琉球医学会 | 論文
- ［総説］生体内における一酸化窒素合成酵素システムの意義の解明
- ［原著］Mechanical Stress regulates Chondrocyte Proliferation and Differentiation during Endochondral Bone Formation
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