[原著］生菌製剤の効果と作用機序に関する研究
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The efficacy of probiotics(prophylactic use of microorgamisms to prevent diseases) which has been empirically used for many decades, was determined by examining the antimicrobial associated proliferation of Clostridium difficile in the intestine and the mechanism of inhibition was studied. Probiotics (C. butyricum spore) was adiministered to the 56 cases out of 109 patients who were on antibiotics therapy. The detection rates of C. difficile in the stool samples(2 specimens from one patient) after antimicrobial therapy were 27.4% (29 specimens out of 106) in the 53 non-probiotics-administered group, and 15.2% (17/112) in the 56 probiotics-administered group(p<0.03). The detection rate of D-1 antigen were 28.3% (30/106), and 17.0% (19/112) (P<0.05) , respectively. Log values of the geometric means of the detected C. difficile count per gram of stool were $5.94 \pm 1.32$ in the probiotics-administered group and $6.97 \pm 1.11$ nonprobiotics-administered group(p<0.02). When C. difficile and C. bulyricum were mixed and cultured in GAM broth, the growth of C. difficile was inhibited after 9 hours of incubation. As the pH tended fowards lower values, the growth also increased. This phenomenon was not seen in a pure culture of C. difficile in GAM broth when pH was lowered by the addition of HCl in contrast to the effect with butyric acid. The increase of antibody-titer was enhanced when mice were immunized with C. difficile together with C. butyricum. These results suggested that C. butyricum inhibit the proliferaton of C. difficile by means of some cellular product and by stimulating the host defence mechanism.
- 琉球医学会の論文
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