［原著］Proteomic Changes in a Squamous Cell Carcinoma Cell Line Induced by an Effector Kinase of a Small G Protein Rap 2

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The Traf2- and Nck-interacting kinase(TNIK) is a downstream effector of a Ras-like small G protein Rap2 identified by us. Very little is known about TNIK's roles in physiological or pathological cellular processes．To examine the effect of activated TNIK in cancer cells, over-expression of TNIK was induced by the use of TetOffsystem in a squamous cell carcinoma cell line. Global protein expression profiling was carried out by Ettan TM twodimensional fluorescence difference gel electrophoresis(2-D DIGE). Proteins were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. By comparlng the cells with or without over－expressed TNIK, 2-D DIGE revealed up-regulation of three proteins and down-regulation of six proteins by TNIK.Peptide mass fingerprinting with MALDI-TOF mass spectrometry identified five out of these nine proteins：isovaleryl coenzyme-A dehydrogenase (up-regulated 2.46-fold, p<0.05); Rho GDP dissociationin hibitor alpha(down-regulated2.35-fold, p<0.05); stomatin-like protein 2 (down-regulated 1.57-fold, p<0.05); ribosomal protein,mitochondrial,S22（down-regulated 1.78-fold,p<0.05); Ornithine aminotransferase (down-regulated 1.34-fold, p<0.05). Three down-regulated proteins, Rho GDP dissociation inhibitor alpha, stomatin1ike protein 2, and ornithine aminotransferase were candidates for cancer prognostic marker or target proteins for chemotheraputic drug development.
琉球医学会の論文