Slow-freezing Injuryによる細胞死滅の数学的モデル化の提案 : 最低到達温度に対する生存曲線の反応速度論的モデルの展開

概要

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The reaction kinetic model was so far proposed by the authors to simulate the cell survival curve as a function of cooling rate under the extracellular freezing in cryopreservation of cells. In this study, the similar mathematical model has been applied and developed to the cell survival curve as a function of end temperature under the slow freezing, where the slow-freezing injury was caused by extracellular freezing w/o intracellular freezing. The model constant, kinetic constant for cell damage and death was determined from inverse problem analysis on the basis of experiment of human erythrocytes to investigate the temperature dependence of the kinetic constant. The result shows that the reaction kinetic model is appropriate for the cell survival curve as a function of end temperature.
2014-04-15