昇温過程におけるイオン液体-水混合溶液中のLysozymeの立体構造変化に関する研究

概要

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Structural modifications of lysozyme in aqueous 1-butyl-3-methylimidazolium nitrate ([bmim][NO_3]) solutions (X=10 and 20 mol%IL) upon heating from -196℃ have been investigated by FTIR spectroscopy. From FTIR spectral analyses, the increment of disordered structure due to the unfolding and the decrease of the α-helical structure of lysozyme occurred on going from liquid to glassy states at both IL concentrations. Remarkably, lysozyme structure at X=10 showed an irreversible change, whereas that at X=20 showed a reversibility with the change of temperature. The difference in the lysozyme structural changes between X=10 and 20 may be due to the difference of the solution structure in the aqueous IL solutions. Our results suggest that high IL concentration such as X=20 has a possibility of a new simple temperature-controlled type cryoprotectant for protein.
低温生物工学会の論文
2013-10-15