DNA配列によるケイヒ基原植物の鑑別

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Cinnamon Bark is the bark of the trunk of Cinnamomum cassia Blume (Lauraceae), or such bark from which a part of the periderm has been removed, which is designated in the Japanese Pharmacopoeia Sixteenth Edition. It is used in many stomachics of Kampo formulas, and also used as a spice which flavor is unique and favoured around the world. Origin plant species of Cinnamon Bark has some relative species, and they are used for medicinal and food purposes in many countries. It is difficult to distinguish C.cassia from other species only by appearance and taste when they are in a form of powder which is one of the most common styles for use. In order to develop methods for identification of origin plant of Cinnamon Bark, we tried some procedures using DNA sequences. Fresh leaves whose origin plant species were known were used to search for suitable DNA regions to distinguish C.cassia, and rbcL region was found. A combination of PCR amplification with restriction enzyme digestion of DNAs from either fresh leaves or powder could distinguish C.cassia from others. The method we developed in this research could be one of the solutions for the difficulties of DNA extraction and PCR amplification of some crude drugs because of their viscous liquid. It worked well for the samples whose DNA was fragmented by heat and dry. So this method may support the identification of other origin plants which were difficult to be distinguished by conventional simple DNA sequencing method.
2013-04-22

DNA配列によるケイヒ基原植物の鑑別

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