リモネン合成酵素の立体選択性逆転を目指したミュータジェネシス

概要

論文の詳細を見る
Limonene is one of the most common monoterpenes found in essential oils, and has two enantiomer forms, (+)-(4R)(=d) and (-)-(4S)(=l). They are stereoselectively synthesized by different enzymes in most cases. Sequence similarities of these enzymes in the closely related species are generally very high, which led to a calculation that stereocontrol is regulated by a small number of amino acids. Attempts to clarify amino acid residues which control limonene synthase stereochemistry by site-directed mutagenesis on d- and l-limonene synthases were performed, and some mutants showed a remarkable decrease in product amount. The 3D-structure models of these mutants indicate that their mutations are located proximate to the reaction pocket. After many attempts, a mutant bearing a combination of certain mutations on d-LS produced more l-form than d-form.
日本生薬学会の論文
2009-08-20