Complementary function of mitogen-activated protein kinase Hog1 from Trichosporonoides megachiliensis in Saccharomyces cerevisiae under hyper-osmotic stress(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
A (TmHog1) gene encoding a mitogen-activated protein kinase (MAPK) homologous to Saccharomyces cerevisiae Hog1 (ScHog1) involved in hyper-osmotic stress signaling was isolated from Trichosporonoides megachiliensis SN-124A, an erythritol-producing yeast. Although TmHog1, like other Hog1 homologs, encoded a kinase catalytic domain containing TGY motif, it was 50-60 amino acid residues shorter than the ScHog1. A TmHog1 transgene rescued the osmotic sensitivity and glycerol production defect of S. cerevisiae hog1Δ, a highly osmo-sensitive strain that does not produce glycerol, a compatible solute, during osmotic stress. Functional analyses of chimeric Hog1 proteins constructed from ScHog1 and TmHog1 sequences indicated that the C-terminal region of TmHog1 is more effective for glycerol biosynthesis than ScHog1 under osmotic stress.
- 公益社団法人日本生物工学会の論文
- 2013-02-00
著者
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Shima Jun
Research Division Of Microbial Sciences Kyoto University
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Shima Jun
Research Division of Microbial Sciences, Kyoto University
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Kobayashi Yosuke
College of Bioresource Sciences, Nihon University
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Koyama Yoshiyuki
College of Bioresource Sciences, Nihon University
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Kasumi Takafumi
College of Bioresource Sciences, Nihon University
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Ogihara Jun
College of Bioresource Sciences, Nihon University
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Yoshida Junjiro
College of Bioresource Sciences, Nihon University
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Tanaka Yosuke
College of Bioresource Sciences, Nihon University
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Kato Jun
College of Bioresource Sciences, Nihon University
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