Physiological Relevance of Hydrolysis of Atrial Natriuretic Peptide by Endothelin-Converting Enzyme-1
スポンサーリンク
概要
- 論文の詳細を見る
Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound metalloprotease that cleaves biologically inactive big endothelin-1 (ET-1) into active ET-1. ET-1 is involved in the cardiovascular homeostasis and the development of cardiovascular diseases including pulmonary arterial hypertension and heart failure. Atrial natriuretic peptide (ANP) is an endogenous hormone that is released from the heart in response to myocardial stretch and overload. ANP was shown to be hydrolyzed by neutral endopeptidase 24.11 (NEP) which shares important structural features with ECE-1. Previous in vitro studies using recombinant soluble ECE-1 suggested that ECE-1 cleaved several biologically active peptides including ANP in addition to big ET-1. However, physiological relevance of ANP-degrading activity by ECE-1 has stayed unclear. Here, we aimed to investigate whether endogenous ECE-1 is able to hydrolyze ANP using live-cell based assay and ECE-1–deficient mice. Chinese hamster ovary (CHO) cells, which lack detectable levels of ECE activity, degraded ANP in the medium efficiently when transfected with ECE-1 cDNA. ANP peptide contents in the E14-15 embryos were significantly higher in ECE-1+/– mice compared with ECE-1+/+ mice. These observations strongly suggest that ECE-1 is involved in the physiological degradation of ANP in vivo. Thus, pharmacological inhibition of ECE-1 may provide a novel strategy to treat various cardiovascular diseases by suppressing and potentiating the ET and ANP pathway, respectively.
- 神戸大学の論文
- 2012-00-00
著者
-
Emoto Noriaki
Division Of Cardiovascular And Respiratory Medicine Kobe University Graduate School Of Medicine
-
Yagi Keiko
Department Of Clinical Pharmacy Kobe Pharmaceutical University
-
Hirata Ken-ichi
Division Of Cardiovascular Medicine Department Of Internal Medicine Kobe University Graduate School
-
Suzuki Yoko
Department Of Cardiology National Maiduru Hospital
-
Nakayama Kazuhiko
Division Of Cardiovascular Medicine Department Of Internal Medicine Kobe University Graduate School Of Medicine
-
Vignon-Zellweger Nicolas
Department of Clinical Pharmacy, Kobe Pharmaceutical University
-
Emoto Noriaki
Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
-
Suzuki Yoko
Department of Clinical Pharmacy, Kobe Pharmaceutical University
関連論文
- Comparison by Optical Coherence Tomography of Paclitaxel-Eluting Stents With Sirolimus-Eluting Stents Implanted in One Coronary Artery in One Procedure : 6-Month Follow-up
- STELLIUM 1 : First-In-Man Follow-up Evaluation of Bioabsorbable Polymer-Coated Paclitaxel-Eluting Stent
- OJ-251 Requirement of Small G Protein Rap1 and Rap1-binding Protein Afadin in Endothelial Cells for the Regulation of Angiogenesis(OJ42,Peripheral Circulation/Vascular Disease (Pathophysiology, Basic) (H),Oral Presentation (Japanese),The 73rd Annual Scien
- Endothelial Lipase Gene Polymorphism is Associated With Acute Myocardial Infarction, Independently of High-Density Lipoprotein-Cholesterol Levels
- Beneficial Effects of Exogenous Tetrahydrobiopterin on Left Ventricular Remodeling After Myocardial Infarction in Rats : The Possible Role of Oxidative Stress Caused by Uncoupled Endothelial Nitric Oxide Synthase
- Whole Body Bioimpedance Monitoring for Outpatient Chronic Heart Failure Follow up
- Prognostic Factors in Childhood Acute Lymphoblastic Leukemia in Japan
- Dilated phase of hypertrophic cardiomyopathy caused by Fabry disease with atrial flutter and ventricular tachycardia
- Knockout of Endothelin-1 in Vascular Endothelial Cells Protects Against Insulin Resistance Induced by High-Salt Diet in Mice
- PE-312 Endothelial Cell-derived Endothelin-1 Regulates Proteinuria and Glomerular Barrier Function in Diabetic Mice(PE053,Peripheral Circulation/Vascular Disease (Therapy), Kidney/Renal Circulation/CKD (H),Poster Session (English),The 73rd Annual Scientif
- PE-270 Impairment of Endothelial Function in Atherosclerotic Mice Lacking Vascular Endothelial Endothelin-1(PE046,Atherosclerosis (Basic, Clinical/Diagnosis) (IHD),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Soc
- PE-189 Endothelin Converting Enzyme-1 Inhibition Augments Arteriogenesis in Streptozotocin Induced Type 1 Diabetic Hindlimb Ischemia Model(PE032,Peripheral Circulation/Vascular Disease (Pathophysiology, Basic) (H),Poster Session (English),The 73rd Annual
- OE-058 Disruption of Cry genes Exaggerate Neointimal Formation in Mouse Flow Cessation Model(OE10,Atherosclerosis, Basic 1 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 1 Endothelial Cell-derived Endothelin-1 Promotes Cardiac Fibrosis in Diabetic Heart through Stimulation of Endothelial to Mesenchymal Transition(Young Investigator's Award for International Students Finalists Lecture (YIA International),The 73rd Annual Sc
- PJ-175 Endothelin Converting Enzyme-1 Heterozygous Mice Augment Ischemic Induced Angiogenesis Recovery in Streptozotocin Induced Type1 Diabetic Hindlimb Ischemia Model(Regeneration(angiogenesis/myocardial regeneration)(04)(M),Poster Session(Japanese),The
- OE-223 Genetic Inactivation of Vascular Endothelial Cell Endothelin-1 in Mice is Protective against Diabetic-Induced Renal Injury(Kidney/Renal circulation(01)(H),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Soc
- OE-139 Preservation of Cardiac Function in Diabetic Mice with Specific Knockout of Endothelin-1 in Vascular Endothelial Cell(Cardiac function, basic/clinical(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation
- OE-116 Vascular Endothelial Cells-derived Endothelin-1 regulates PECAM-1 and ICAM-1 expression after endothelial injury in vivo : Role of ET-1 in vascular inflammation(Infection/Inflammation/Immunity(01)(H),Oral Presentation(English),The 72nd Annual Scien
- 5 Disruption of Endothelial Cells-Specific Endothelin-1 Gene Reduced Matrix Deposition in Atherosclerotic Lesion of Innominate Artery in Apolipoprotein E-Null Mice(Young Investigator's Award for International Students Finalists Lecture (YIA International)
- Value of Convex-Type ST-Segment Elevation and Abnormal Q Waves for Electrocardiographic-Based Identification of Left Ventricular Remodeling in Hypertrophic Cardiomyopathy
- Plasma Tetrahydrobiopterin/Dihydrobiopterin Ratio : A Possible Marker of Endothelial Dysfunction
- Overexpression of Endothelial Nitric Oxide Synthase Attenuates Cardiac Hypertrophy Induced by Chronic Isoproterenol Infusion
- Factors That Influence Measurements and Accurate Evaluation of Stent Apposition by Optical Coherence Tomography : Assessment Using a Phantom Model
- Role of Endothelial Lipase in Plasma HDL levels in a Murine Model of Hypertriglyceridemia
- Endothelial Lipase is Increased by Inflammation and Promotes LDL Uptake in Macrophages
- Targeted Disruption and Overexpression of Endothelial Cell-Derived Lipase Modulates Plasma HDL and LDL Cholesterol Levels
- Expression of G2A, a Receptor for Lysophosphatidylcholine, by Macrophages in Murine, Rabbit and Human Atherosclerotic Plaques
- In Vivo Angiographic Detection of Vascular Lesions in Apolipoprotein E-Knockout Mice Using a Synchrotron Radiation Microangiography System
- A HMG-CoA Reductase Inhibitor Reduces Hypertensive Nephrosclerosis In Stroke Prone-SHR
- A HMG-CoA Reductase Inhibitor Reduces Stroke Events and Mortality In Stroke-Prone Spontaneous Hypertensive Rats
- Supplement of tetrahydrobiopterin inhibits atherosclerosis in apoE-deficient mice
- Overexpression of endothelial nitric oxide synthase accelerates atherosclerotic lesion development in apoE-deficient mice
- Interaction of Oxidative Stress and Inflammatory Response in Coronary Plaque Stability : Important Role of C Reactive Protein
- Role of Angiotensin and the Clock System in the Circadian Regulation of Plasminogen Activator Inhibitor-1
- FRS-112 Pressure-overload Induced Hypertrophied Hearts Displayed Regional Differences in Cross-bridge Dynamics : Evidence from X-Ray Diffraction Study Using Synchrotron Radiation(New Concepts for Assessing Cardiac Function(M),Featured Research Session,The
- Inhibition of Endogenous Nitric Oxide Synthase Augments Contractile Response to Adenylyl Cyclase Stimulation Without Altering Mechanical Efficiency in Patients With Idiopathic Dilated Cardiomyopathy
- PE-209 VEGF Upregulation In Mice Lacking Endothelin-1 In Vascular Endothelial Cell Compensates the Lack of Heart Vascularization on the Expense of Inflammation(Cardiac hypertrophy, basic/clinical-2, The 71st Annual Scientific Meeting of the Japanese Circu
- PE-079 ACE Inhibitors (ACE-I) and Angiotensin Receptor Blockers (ARB) Have Different Effects on the Circadian Expression of Plasminogen Activator Inhibitor-1 (PAI-1)(Thromboembolism/Antithrombotic therapy/Thrombolysis-2, The 71st Annual Scientific Meeting
- OE-390 Attenuation of Cardiac and Renal Diabetic Complication in Mice with Specific Knockout of Endothelin-1 in Vascular Endothelial Cell(Diabetes/Obesity/Metabolic syndrome-02, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Decreased Ornithine Decarboxylase Activity in the Kidneys of Dahl Salt-Sensitive Rats
- Expressional Changes of the Vascular Antioxidant System in Atherosclerotic Coronary Arteries
- Dose Control Study on Fentanyl Patch Using Two Types of Covering Material
- Dominant Negative c-Jun Inhibits Platelet-Derived Growth Factor-Directed Migration by Vascular Smooth Muscle Cells
- Delayed Neointimalization on Sirolimus-Eluting Stents : 6-Month and 12-Month Follow up by Optical Coherence Tomography
- OE-238 Inhibition of Vascular Endothelial Endothelin-1 Reduced Inflammatory Cells Recruitment and Neointimal Formation after Flow Cessation(Atherosclerosis, basic-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-272 Knockout of Endothelin-1 in Vascular Endothelial Cell, through Impaired Angiogenesis, resulted in reduced Adipose Tissue Mass and Lower Blood Pressure(Hypertension, basic-1 (H) OE46,Oral Presentation (English),The 70th Anniversary Annual Scientific
- FRS-046 Genetic Inactivation of Vascular Endothelial Cell Endothelin-1 is Protective Against High-fat Diet Induced Obesity and Metabolic Syndrome(Progress in Preventive Medicine-1 (H) FRS10,Featured Research Session,The 70th Anniversary Annual Scientific
- Endothelial Cell-specific Endothelin-1 Knockout Mice Have Impared Cardiac Function and Increased Cardiac Fibrosis under the Chronic Pressure Overload Condition (Heart Failure, Basic 7 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Soc
- Role of Endothelial Cells-derived Endothelin-1 in Preventing Hypoxia-induced Pulmonary Hypertension in Mice : A Conditional Knockout Study(Pulmonary Circulation 3 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- The Role of Endothelin-1 in Vascular Endothelial Cells in insulin Resistance Induced by a High-salt Diet(Diabetes/Obesity 5 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Normal Phenotype of Endothelin-Converting Enzyme-1 Heterozygous Mice Suggest Functional Compensation by Renin-Angiotensin System(Neurohumoral Factors 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Genetic Inactivation of Vascular Endothelial Endothelin-1 in Mice is Protective against Angiotensin II-induced Hypertension, Vascular Remodeling and Cardiac Fibrosis(The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PE-375 Endothelin-1 Partially Mediated Angiotensin II-induced hypertension, Cardiac Fibrosis and Body Weight Loss(Hypertension, Basic 4 (H) : PE64)(Poster Session (English))
- Proapoptotic Effect of a Dietary Supplement : Water Soluble Chitosan Activates Caspase-8 and Modulating Death Receptor Expression
- Low plasma levels of hemostatic proteins during the induction phase in children with acute lymphoblastic leukemia : A retrospective study by the JACLS
- Translational Research of Endothelin System : A Growing Body of Clinical Work Driven by High Quality Basic Science(Translational Research in Cardiovascular Disease, The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Chitosan Induces Apoptosis via Caspase-3 Activation in Bladder Tumor Cells
- Autologous Hematopoietic Stem Cell Transplantation for 3 Patients with Severe Juvenile Rheumatoid Arthritis
- Inhibition of RhoA or Rac1? Mechanism of Cholesterol-Independent Beneficial Effects of Statins
- Molecular Cloning and Expression Analysis of Rat Endothelial Cell-Derived Lipase
- BTG1, an anti-proliferative gene, regulates endothelial cell growth and differentiation during angiogenesis
- Role of Endothelial Cell-Derived Lipase in Monocyte Adhesion to Endothelial Cells
- PE-397 The Role of the Endogenous Biological Clock in the Regulation of Circadian Rhythm of Cardiovascular System(Hypertension, basic-2 (H) PE67,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OE-250 Role of Endothelin-Converting Enzyme-1 and Bradykinin in Hypoxia-induced Pulmonary Hypertension(Pulmonary circulation-1 (H) OE42,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- The Central Pacemaker Plays a Role for Stabilizing the Circadian Rhythm of Cardiovascular Function(Hypertension, Basic 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PE-373 The functional role of the central and peripheral clock for regulating blood pressure.(Hypertension, Basic 4 (H) : PE64)(Poster Session (English))
- PE-352 Clock Gene Expression in Human Leucocytes as a Marker for Circadian Rhythm(Cardiovascular Pharmacology, Basic/Clinical 3 (H) : PE60)(Poster Session (English))
- OE-307 Endothelial cell-specific inactivation of ET-1 protects mice against pulmonary hypertension and right ventricular hypertrophy(Hypertension, Basic 3 (H) : OE39)(Oral Presentation (English))
- Dissection of the Central and Peripheral Clock in the Role for Regulating Cardiovascular Function
- Activation of the Renin-Angiotensin System in Endothelin-Converting Enzyme-1 Deficient Mice
- The Beneficial Effects of CGS26303 (ECE/NEP Dual Inhibitor) in Dahl Salt-Sensitive Rat Compared with ACE Inhibitor and NEP Inhibition
- Characterization of the Expression of NHE-3 Gene as an Ouput Gene of Clock
- PJ-084 Circadian expression of plasminogen activator inhibitor-1 (PAI-1) is regulated in a different manner among tissues of mice(Thromboembolism/Thrombolysis 2 (IHD) : PJ14)(Poster Session (Japanese))
- The Amplitudes of Circadian Expression of Clock Genes Are Decreased in Dahl-Salt Sensitive Rats Fed a High Salt Diet
- Isolation and Characterization of Output Genes under the Regulation of Biological Clock in Vascular Smooth Muscle Cells
- Human ECE-1a, whose expression is enhanced in failing heart, presents at the cell surface as well as in intracellular when transfected CHO
- Circadian Gene Expression in Dahl-Salt Sensitive Rat
- Molecular identification and characterization of a novel gene that is upregulated in obese mouse heart
- Circadian Oscillation in the Expression of Plasminogen Activator Inhibitor Type-1 Gene in Vascular Smooth Cells is Induced by Angiotensin II
- 1 Dissection of Roles of Endothelin-1 in the Cardiovascular System : Lessons from Genetically Modified Mice(The Role of the Endothelin System in Cardiovascular Diseases, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Serum beta2-microglobulin concentration as a novel marker to distinguish levels of risk in acute heart failure patients
- CD34^+ progenitor cell transplantation from two HLA-mismatched healthy fathers to two infants with severe aplastic anemia
- 3 The Role of Endothelial Lipase in HDL Metabolism and Atherosclerosis : A New Therapeutic Target for Raising HDL-Cholesterol(Hypoalphalipoproteinemia, Its Pathogenesis and Treatments, The 71st Annual Scientific Meeting of the Japanese Circulation Society
- OE-058 Endothelial Lipase is Responsible for the Low Selrum HDL Level in the Chemically Induced Murine Model of Hyperlipidemia(Lipid disorders-1 (H) OE10,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulatio
- Impact of Cytochrome P450 2C19^*2 Polymorphism on Intra-Stent Thrombus After Drug Eluting Stent Implantation in Japanese Patients Receiving Clopidogrel
- Isolated true parachute mitral valve in an asymptomatic elderly patient
- 1 Molecular Mechanisms of the Regulation in Diurnal Blood Pressure Change by Biological Clock(Symposium 2 (SY2) (H) : Hypertension : From Molecular Mechanisms to Tailored Therapy)(Special Program)
- Ability of Optical Coherence Tomography to Visualize the Entry Port of Spontaneous Coronary Artery Dissection
- Effect of Left Ventricular Dyssynchrony on Cardiac Sympathetic Activity in Heart Failure Patients With Wide QRS Duration
- Impact of Left Ventricular Afterload on Longitudinal Dyssynchrony in Patients With Severe Aortic Stenosis and Preserved Ejection Fraction
- Tissue Doppler Imaging-Derived Myocardial Acceleration During Isovolumetric Contraction Predicts Pulmonary Capillary Wedge Pressure in Patients With Reduced Ejection Fraction
- A case of transient myocardial dysfunction with electrocardiographic abnormality due to acute carbon monoxide poisoning
- Impact of Stent Platform of Paclitaxel-Eluting Stents : Assessment of Neointimal Distribution on Optical Coherence Tomography
- Efficacy of Right Ventricular Free-Wall Longitudinal Speckle-Tracking Strain for Predicting Long-Term Outcome in Patients With Pulmonary Hypertension
- Physiological Relevance of Hydrolysis of Atrial Natriuretic Peptide by Endothelin-Converting Enzyme-1
- Rhodamine-Labeled LDL as a Tool to Monitor the Lipoprotein Traffic in Experimental Model of Early Atherosclerosis in Mice
- Noninvasive and Simple Assessment of Cardiac Output and Pulmonary Vascular Resistance With Whole-Body Impedance Cardiography Is Useful for Monitoring Patients With Pulmonary Hypertension
- Localization and Characterization of a Novel Secreted Protein, SCUBE2, in the Development and Progression of Atherosclerosis
- A New Class of Drug for Pulmonary Arterial Hypertension:– Can a Rho-Kinase Inhibitor Break the Stagnation in Treating It? –
- Noninvasive and Simple Assessment of Cardiac Output and Pulmonary Vascular Resistance With Whole-Body Impedance Cardiography Is Useful for Monitoring Patients With Pulmonary Hypertension