簡易電気泳動と活性色素を用いた「酵素の働き」に関する実験の教材化

概要

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The gel electrophoresis is a very important method of analysis in the research of molecular biology andbiochemistry, and the method to separate and to analyze the protein is being executed in the student experimentof this college now. Therefore, the experience of the technique seems to be meaningful for junior high schoolstudents.However, it is difficult to include such experiments in the school experience and public lecture for junior highschool student because of safety concerns, the expensive materials and experimental equipment and timeconsumed.This report introduces an electrophoresis using horizontal agar gels for the detection of enzyme activity.Activity of the enzyme was rapidly and easily visualized on the gels. The total time required for the experimentsis less than 1 hour. The experiments proposed here are the convenient teaching materials to acquire molecularbiological knowledge and techniques.
福島工業高等専門学校の論文