DNA Slot Hybridization法による小柴胡湯およびその単味生薬の抗EBウイルス活性の検討

概要

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A nucleic acid hybridization method for Epstein-Barr virus (EBV) DNA content is described. Cell lysates were prepared and the DNA was fixed to a nitrocellulose membrane. After hybridization to biotin-labeled EBV DNA fragment, the extent of hybridization was monitored by using a densitometer. By the statistical analysis of the serial dilutions of cell samples, their viral genome content can be estimated. This method was proved to be a simple and sensitive assay system for the screening of potential antiviral drugs having an anti-EBV activity. When crude extracts of Syo-saiko-to and its components were evaluated by this method, the selectivity indexes, based on the ratio between the 50% cytotoxic dose and the 50% antiviral effective dose, of Syo-saiko-to, Penelliae Tuber and Bupleuri Radix were 4.3, 3.3 and 3.2, respectively. Other extracts were not found to be active.
日本生薬学会の論文
1989-06-20