サケ由来DNAを素材にした新規生体材料の開発

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The DNA/cationic amphiphilic lipid complexes, a DNA/chitosan complex and a DNA/protamine complex were prepared by the reaction of cationic materials (amphiphilic lipid, chitosan, and protamine) with salmon-serum DNA in an aqueous solution to apply DNA for biomaterials. All complex powder was water insoluble. Some DNA/cationic amphiphilic lipid complexes became films (DNA film) by casting complexes from an organic solvent. A mild tissue response of the DNA films was confirmed after implantation into the skin on the back of rats and DNA films biodegraded 3 days after being implanted. The implanted area was replaced with scar tissues that consisted of mature collagen fibers. The DNA/chitosan complexes were porous. The porosity of the complexes was decreased by rinsing them with a PBS buffer. In a cell culture test using MG-63 osteoblast-like cells, the DNA/chitosan complex showed less cytotoxicity than chitosan alone. The complex caused only a mold tissue response when implanted subcutaneously in the backs of rats. The complex biodegraded 5 days after being implanted. The DNA/chitosan complex disk could be fabricated by rinsing with a PBS buffer. It was found that the biodegradation of fabricated disk was delayed without any serious tissue responses in vivo. The DNA/protamine complex was porous. The complex powder became a fluid paste by kneading with water and the complex paste showed less porosity than the complex powder. In a cell culture test using MC 3T3-E1 osteoblast-like cells, the DNA/protamine complex showed less cytotoxicity than protamine alone. The complex powder and paste showed mold tissue responses when implanted subcutaneously in the backs of rats. Both biodegraded approximately 10 days after being implanted. It seems from the result in vitro and in vivo tests that the complex paste may differentiate into osteoblasts under osteogenic induction. From the results of these studies it is considered that DNA/cationic amphiphilic lipid complexes, DNA/chitosan complexes and DNA/protamine complexes have the potential to be used for biomaterials such as for a scaffold and drug delivery.
2011-09-30

著者

福島忠男
福岡歯科大学再生医学研究センター

サケ由来DNAを素材にした新規生体材料の開発

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