T0201-2-3 伸縮による間葉系幹細胞の腱細胞への分化([T0201-2]細胞の構造と流れにおけるマイクロ・ナノスケール解析(2))

概要

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Adult somatic cells are influenced by mechanical stresses such as shear stress, pressure and etc. Cultured cells are also affected by these stresses. Especially, many researches have been reported that mechanical stretch can regulates the differentiation of bone marrow mesenchymal stem cells (BMSCs) into tenocytes. In this study, we examined the effect of mechanical stretch under different extension ratio of 5% and 10%, respectively. The experiment results show that the protein expressions of collagen types I, tenascin-C and RUNX2 by 10% stretch are more effective regulated than by 5% stretch. In addition, the gene expressions of collagen types I and tenascin-C are up-regulated. We suggest that these results were caused because the magnitude of 5% stretch was insufficient to induce differentiation into tenocytes. Therefore, we concluded that mechanical stretch under different extension ratio causes the different responses, and 10% stretch is more adequately for the differentiation into tenocytes than 5% stretch.
一般社団法人日本機械学会の論文
2010-09-04