低真空走査型電子顕微鏡による室内塵性ダニ類観察のための簡易凍結乾燥手法

概要

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A new simple technique for examining microstrctures of house mites, low-vacuum SEM freeze drying, was applied for observation of Dermatophagoides puteronyssinus, Chelacaropsis moorei and Tyrophagus putrescentiae, although those mites had been previously examined by a complicated SEM technique. Water with a surface active agent was dropped on a specimen stub to which a piece of double-faced carbon tape was attached. A living or dead mite was dropped into the water on the stub and frozen by pouring liquid nitrogen around the stub. The stub is then set to a specimen holder in low-vacuum SEM to dehydrate the specimen in a chamber. Mites could be observed at this stage. Besides, the specimen could be observed in high-vacuum SEM after it was evaporated using gold. This new technique shortened the time for dehydration with less failure in specimen-preparation, and showed favorable observation compared with previous complicated techniques.
日本家屋害虫学会の論文
1995-12-20