実験的糖尿病ラットにおける細胞植え込み型人工膵島(bio-artificial endocrine pancreas)に関する研究

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This experiment was designed to investigate whether MIN6-cells, which are derived from transgenic mouse insulinoma cells, could be a useful tool for a bioartificial endocrine pancreas (Bio-AEP), when enclosed in a diffusion chamber constructe of a semipermeable membrane (pore size 0.2 μm) and a silicon ring. Six streptozotocin (STZ)-induced diabetic rats were each implanted with a Bio-AEP containing MIN6-cells, but without any immunosuppression therapy. Three STZ-induced diabetic rats were implanted with a diffusion chamber without the MIN6-cells, as a sham operation. In three of the six STZ-diabetic rats with a Bio-AEP, fasting blood glucose levels were normalized immediately and remained within normal range until the 32th week after implantation (good control). In the other three rats, with Bio-AEPs, the blood glucose level rose gradually from the 24th week after implantation (poor control). The fasting blood glucose levels in the three rats in the sham operation group were higher than 600 mg/dl, immediately after the implantation. Thirty-five weeks after implantation, the Bio-AEPs were removed from the nine diabetic rats. Subsequently, the blood glucose levels of the three rats in the good control groups, rose quickly, whereas those of the rats in the sham operation and poor control groups, were sustained above 600 mg/dl, which was as high as the level before Bio-AEP removal. The Bio-AEP removed from the rats in the good control group were intact. In contrasta, in the poor control group, either the nuclepore membrane or the silicon ring had been damaged and the MIN6-cells had disapperared due to lysis. No relationship was found between the l0ocation of the Bio-AEP in the abdominal cavity and the blood glucose levels. We conclude that the Bio-AEP containing MIN6-cells is useful for a long-term normalization of blood glucose levels in diabetic rats.
東京女子医科大学の論文
1996-12-25

実験的糖尿病ラットにおける細胞植え込み型人工膵島(bio-artificial endocrine pancreas)に関する研究

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