INHIBITION OF ACTO-HEAVY MEROMYOSIN AND ACTOMYOSIN Mg^<2+>-DEPENDENT ADENOSINE-5'-TRIPHOSPHATASE FROM RABBIT SKELETAL MUSCLE BY TUBULIN FROM RAT BRAIN

概要

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Tublin from rat brain microtubules inhibited actin-activated Mg-ATPase of heavy meromyosin (HMM) in the same way as tubulin inhibited that of myosin (our previous report [1979] Life Sci. 24, 1683-1690). The apparent affinity of HMM for actin was not altered by tubulin. At low ionic strengthsm tubulin did not affect either the turbidity or the viscosity of HMM and acto-HMM, although it inhibited the turbidity increase of actomyosin by ATP. The inhibition of HMM ATPase was only slightly changed by the order of addition of HMM, actin, and tubulin, whereas the order affected marked the extent of inhibition of acto-myosin ATPase. The inhibition diminished when microtubule-associated proteins (MAPs) were preincubated under such conditions that tubulin was bound to MAPs before forming other complexes.
名古屋市立大学の論文
1982-07-30