MORPHOLOGICAL CHANGES BY TREATMENT FACTORS OF COATED VESICLES FROM BOVINE CEREBRUM

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The morpfological changes of the coated vesicles from bovine cerebrum were investigated under a variety of conditions including the ones used conventionally for the preparation of electron microscopic specimens. Although the vesicle has generally been thought to appear as a vesicle encased in a basket-like coat both in situ and after isolation, the appearance has gradually been noted not to be invariable. Most interestingly in our experiment, imidazole-HC1 buffer (pH 7.4) preserved the basket-like coat structure, whereas the coated vesicles were compacted to form smooth-surfaced plain vesicle-like particulates in Tris-HC1, histidine-HC1, etc. at the same pH. The vesicles that had been compacted in cacodylate buffer (pH 7.4) converted into the initial basket-like structure after the addition of glutaraldehyde. Since even the preparatory condetions for electron microscopic examination could thus affect the appearance of the coated vesicle, one has to be extremely cautious in interpretation of the morphological data with regard to the occurrence and localization of the vesicles.
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