78.キュウリ芽生えの重力形態形成に伴うIAA動態変化の解析(口頭発表)

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When cucumber seeds are germinated in a horizontal position, resulting seedlings develop a specialized protuberance, termed the peg, on the lower side of the root-hypocotyl transition zone (TR zone), because gravistimulation suppresses peg formation on the upper side of it. We have shown that 2min of gravistimulation is sufficient for the suppression of peg formation. Also, we have shown that redistribution of indole-3-acetic acid (IAA) plays an important role in regulation of this gravimorphogenesis. However, we do not know whether auxin redistribute within 2min following gravistimulation. To verify the possibility of this rapid redistribution of IAA upon gravistimulation, we investigated it by immunofluorescence method using anti-IAA monoclonal antibody. When the excised TR zone was treated with IAA, immunofluorescent signals of IAA were detected at higher intensity than those in the IAA-starved segments. These results indicated that our immunofluorescent analysis was valid for monitoring IAA. When cucumber seedlings grown in a vertical position were gravistimulated by orienting them in a horizontal position, distribution of IAA-signals changed within 2min after gravistimulation. The intensity increased in nuclei and cytoplasm of epidermal cells in the lower side of the TR zone, compared to that in the upper side. Thus, IAA redistribution commences to occur within 2min could be responsible for the gravity-regulated suppression of peg formation in cucumber seedlings.
2008-10-06

78.キュウリ芽生えの重力形態形成に伴うIAA動態変化の解析(口頭発表)

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