INV-3 超音波の分子的生体作用と治療応用(招待講演3)

概要

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To understand how therapeutic ultrasound works, understanding its biological effects is deemed necessary. Here, the molecular aspect, particularly on apoptosis induction, gene expression, and gene transfection were investigated. Apoptosis induction was assayed by flow cytometry and by other methods targeting indicators of apoptosis. Gene expressions were evaluated using western blotting, real-time PCR, and micro-array analyses. And gene transfectionwas investigated using luciferase assay and other methods. The results showed that low intensity ultrasound can induce apoptosis in cancer cell lines and that it can be optimized using pulsed ultrasound. Ultrasound resulted to down-regulation or up-regulation of some genes. Of particular interest is the striking up-regulation of heme oxygenase-1 gene, a gene usually associated with oxidative stress. Introducing gene using ultrasound also showed promising results. Membrane damage is pivotal to the bioeffects that modifying the cell membranes either promotes or inhibits the desired effects. In summary, we conclude that ultrasound can be useful in therapy that requires apoptosis induction or gene introduction to cells.
超音波エレクトロニクスの基礎と応用に関するシンポジウム運営委員会の論文
2006-11-15