Binding Properties of GP1 Protein of Borna Disease Virus(Virology)
スポンサーリンク
概要
- 論文の詳細を見る
The surface glycoprotein (G) of Borna disease virus (BDV) plays central roles in the process of viral entry. BDV G is cleaved by cellular furin-like proteases into two components, GP1 and GP2. Although GP1 is involved in the virus entry into cells, the binding activity of GP1 to cells is unknown. Therefore, we expressed the wild-type GP1 and a variety of GP1 deletion mutants that were FLAG-tagged at the C-terminus in human embryonic kidney 293T cells. These proteins were then purified using an anti-FLAG antibody and evaluated for their ability to bind to cell lines. GP1 bound to BDV-permissive cells but not to non-permissive cells. GP1 also inhibited BDV infection via its binding to cells. This binding assay should prove useful to map the receptor-binding domain of BDV.
- 社団法人日本獣医学会の論文
- 2009-02-25
著者
-
Horimoto Taisuke
Division Of Virology Department Of Microbiology And Immunology Institute Of Medical Science The Univ
-
Makino Akiko
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The U
-
Kawaoka Yoshihiro
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The U
-
Kawaoka Yoshihiro
Division Of Virology Department Of Microbiology And Immunology Institute Of Medical Science The Univ
-
Makino Akiko
Division Of Virology Department Of Microbiology And Immunology Institute Of Medical Science The Univ
関連論文
- Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions
- Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the α2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks
- Generation of Vero Cells Expressing Ebola Virus Glycoprotein
- A case of pandemic 2009 influenza A (H1N1) in a patient with HIV infection
- Molecular Aspects of Emerging Negative Strand RNA Viruses
- Binding Properties of GP1 Protein of Borna Disease Virus(Virology)
- Molecular Characterization of the Hemagglutinin and Neuraminidase Genes of H5N1 Influenza A Viruses Isolated from Poultry in Vietnam from 2004 to 2005
- Cell Surface Molecules Involved in Infection Mediated by Lymphocytic Choriomeningitis Virus Glycoprotein
- An H5N1 highly pathogenic avian influenza virus isolated from a local tree sparrow in Indonesia
- Characteristics of Influenza Virus Genome Mutations
- Gingyo Gedokusan vs Oseltamivir for the Treatment of Uncomplicated Influenza and Influenza-like illness : An Open-label Prospective Study
- Antigenic Differences between H5N1 Human Influenza Viruses Isolated in 1997 and 2003