Generation of Vero Cells Expressing Ebola Virus Glycoprotein

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概要

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• To establish replication-incompetent Ebola virus (EBOV) lacking its glycoprotein (GP), we attempted to generate a Vero cell line that constitutively expressed GP. We used a retroviral vector to transduce Vero cells with the EBOV GP gene, resulting in a high expression level of GP on the cell surface. The Vero cells expressing EBOV GP complemented the replication cycle of vesicular stomatitis virus, which lacks the essential viral glycoprotein. This cell line might be useful for basic research on EBOV GP as well as for vaccine production.

• 社団法人　日本獣医学会の論文

著者

• Kawaoka Yoshihiro

  東京大学医科学研究所 ウイルス感染分野

• Makino Akiko

  Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The U

• Kawaoka Yoshihiro

  Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The U

• Kawaoka Yoshihiro

  Division Of Virology Department Of Microbiology And Immunology Institute Of Medical Science The Univ

• Makino Akiko

  Division Of Virology Department Of Microbiology And Immunology Institute Of Medical Science The Univ

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