3次元マイクロアレイ : 乳癌の診断と治療効果予測への臨床応用を目指して

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Estrogen signaling plays crucial roles in genesis and malignant progression of breast cancer. Therefore, it is the most important molecular target for diagnosis and therapy of breast cancer. So far, we investigated the estrogen signaling pathway in breast cancer cells using estrogen-responsive DNA microarray. In this study, we assessed the performance of new microarray technology, 3-dimentinal (3D) microarray, to analyze estrogen signaling in clinical specimens. for the future clinical application. 3D-microarray fixes DNA probes on PamChip, the steric microindentation platform. Therefore, 3D microarray is able to fix DNA probes on wide surface area, and thus it has many advantages such as high sensitivity and reproducibility, in comparison with conventional microarray. Furthermore, this system enables short time and automatic analysis from small samples such as biopsy specimens. First we analyzed the estrogen-responsive expression profile in breast cancer tissue using PamChip fixed DNA probes of estrogen-responsive genes, which previously we had identified. The clustering analysis of the results, this array Chip was able to classify the genes distinctly into ER-positive tissue or -negative tissue. Thereafter, we explored to find the minimum amount of the samples in RNA extraction that can keep reproducibility of the result. In this 3D microarray system, it was proved that the 1×10^4 cells were enough to obtain the reproducibility of the analysis. These results indicate 3D-microarray will be a promising tool which clinically applicable for diagnosis from small amount of biopsy specimens.原著Original Paper
東北大学の論文

3次元マイクロアレイ : 乳癌の診断と治療効果予測への臨床応用を目指して

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