An Alternatively Spliced Site in the Proline-Rich Region of Microtubule–Associated Protein 4 Plays an Important Role in Microtubule Stabilization

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概要

• 論文の詳細を見る

• We previously reported that the microtubule-stabilizing activity of amicrotubule-associated protein (MAP) 4 variant, with a deletion in the Pro-rich region(MAP4-SP), was lower than that of a variant with a full length Pro-rich region (MAP4-LP).However, it remained unclear whether the deletion of the specific site in the Pro-rich regionis responsible for the reduction of the microtubule-stabilizing activity. To answer thisquestion, we examined the microtubule-stabilizing activities of four different MAP4variants, MAP4-SP, MAP4-LP, and two additional MAP4-LP variants lacking a part of theRepeat region, and considered the correlation between the activity and the structure.When microtubules assembled in the presence of each of the MAP4 variants were treatedwith nocodazole for disassembly, the MAP4-SP-induced microtubules were significantly lessstable than the other variant-induced microtubules. Another set of experiments, in whichthe microtubules were allowed to disassemble by dilution, yielded similar results: theMAP4-SP-induced microtubules were significantly less stable than the other variant-inducedmicrotubules. The results clearly indicated that the microtubule-stabilizing activity ofMAP4 depends on the specific, alternatively spliced site in the Pro-rich region.

著者

• KOTANI Susumu

  Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineeri

• Matsushima Kazuyuki

  Department of Biological Sciences, Faculty of Science, Kanagawa University

• Matsushima Kazuyuki

  Department Of Biological Sciences Faculty Of Science-city Kanagawa University

• Kotani Susumu

  Kanagawa Univ. Kanagawa Jpn

• Kotani Susumu

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

• TOKURAKU Kiyotaka

  Department of Chemical Science and Engineering, Miyakonojo National College of Technology

• Matsushima K

  Department Of Biological Sciences Faculty Of Science-city Kanagawa University

• Moriyama Hatsune

  Department of Bioscience and Bioinformatics, Faculty of Computer Science and Systems Engineering, Ky

• Tokuraku K

  Department Of Chemical Science And Engineering Miyakonojo National College Of Technology

• Tokuraku Kiyotaka

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Moriyama Hatsune

  Department Of Bioscience And Bioinformatics Faculty Of Computer Science And Systems Engineering Kyus

• Matsushima Kazuyuki

  Dept. Of Biological Sci. Univ. Of Kanagawa

• Tokuraku Kiyotaka

  Dept. Of Chem. Sci. And Eng. Miyakonojo Nat. Col. Of Tech.

• Kotani S

  Kanagawa Univ. Kanagawa

• Kotani Susumu

  Department Of Biological Sciences Faculty Of Science Kanagawa University

• Matsushima Kazuyuki

  Department Of Biological Sciences Faculty Of Science Kanagawa University

関連論文

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• An Alternatively Spliced Site in the Proline-Rich Region of Microtubule–Associated Protein 4 Plays an Important Role in Microtubule Stabilization
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• Deletion in the Pro-rich Region of Microtubule-associated Protein 4 Influences Its Distribution in Neural Growth Cone
• Comparison of a major heat-stable microtubule-associated protein in HeLa cells and 190-kDa microtubule-associated protein in bovine adrenal cortex.

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