The Smallest Active Fragment of Microtubule-Associated Protein 4 and Its Interaction with Microtubules in Phosphate Buffer
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概要
- 論文の詳細を見る
To analyze the interaction between microtubule-associated protein (MAP) 4 and microtubules physicochemically, a MAP4 active site fragment was designed for nuclear magnetic resonance (NMR) use. The fragment was bacterially expressed and purified to homogeneity. The buffer conditions for NMR were optimized to support microtubule assembly. The fragment was found to bind to microtubules under the optimized buffer conditions.
著者
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Matsushima Kazuyuki
Department Of Biological Sciences Faculty Of Science-city Kanagawa University
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Kotani Susumu
Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin
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Nagase Lisa
Department Of Biological Sciences Faculty Of Science Kanagawa University
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Hashi Yurika
Department Of Biological Sciences Faculty Of Science Kanagawa University
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