臍帯血単核球から血管内皮細胞への分化に伴う接着細胞の細胞形態および抗原の解析

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Endothelial progenitor cells(EPCs) have been found to be generated from peripheral blood, bone marrow, and umbilical cord blood(CB) and to be heterogeneous population, however specific variation in their characteristics remains to be clarified. In this study, when CB mono-nuclear cells(CBMCs) were cultured for 6 weeks, the adherent cells changed in morphology from originally round-shape into spindle-shape to form an apparently homogenous population, quite similar to mature endothelial cells(ECs). However, closer observation of this differentiation process revealed that there was a distinct stage, when the adherent cells showed a quite different morphology from either initial round one or that of ECs. After 3 to 5 days of culture, adherent cells appeared with a morphology quite similar to the EPCs. In addition, in early days of culture along with the differentiation, two distinct populations of EPCs were detected, defined by two-dimensional dot plots (forward scatter versus side scatter) with flowcytometry, namely relatively small cells(S-EPCs) and relatively large cells(LEPCs). S-EPCs were found to express CD34 but not CD14, while the converse was the case for L-EPCs. When CD34+/CD14-幼ells and CD34-/CD14+ cells were isolated from original CBMCs and cultured independently, S-EPCs and L-EPCs were derived from CD34+/CD14- and from CD34-/CD14+ cells, respectively. In conclusion, we have obtained evidence that two distinct populations of EPCs differing in size on two-dimensional dot plots can be identified during differentiation from CBMCs into ECs. Of the two EPCs, S-EPCs originate from CD34+ cells and L-EPCs from CD14+ cells.

臍帯血単核球から血管内皮細胞への分化に伴う接着細胞の細胞形態および抗原の解析

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