リン脂質の過酸化に関する研究 (第1報) : 高速液体クロマトー化学発光による過酸化反応の追跡

概要

論文の詳細を見る
Many reports have pointed out that the peroxidation of phospholipid might promote ageing and cause cancer. Is is very important to follow up the peroxidation reaction in vivo. But as it is very difficult, I tried to follow up the reaction in vitro using high-performance liquid chromatography (HPLC) and chemiluminescence (CL) system as a basic study. Phospholipid was peroxidized by several methods and the products i.e,phospholipid hydroperoxides were separated with HPLC and detected with the post column method of CL using cytochrome C and luminole. T-butylhydroperoxide, which was used as a standard substant of peroxides, was detected at picomole level with the method of HPLC-CL. The most effective method for peroxidation was the photosensitized peroxidation using methyleneblue. This reaction was inhibited by thiourea, so it was verified that the peroxidation had occured. And a slight peroxidation was recognized after the irradiation of ultraviolet light.
松山東雲女子大学・松山東雲短期大学の論文