(-)-Epigallocatechin 3-O-gallate (EGCG) -induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells
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概要
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(-)-Epigallocatechin 3-O-gallate (EGCG), a major catechin in green tea, suppresses renal failure in animals, and inhibits the growth of mesangial cells and opossum kidney proximal tubular cells. In addition, gallic acid, a structural constituent of this catechin, induces apoptosis in tumor cell lines. However, the effects of catechins on renal fibroblastic cells have not been investigated. In this experiment, the growth of normal rat kidney interstitial fibroblast (NRK-49F) cells was significantly inhibited by EGCG at concentrations higher than 6.25 μM, and almost completely inhibited at concentrations over 200 μM. The numbers of in situ end-labeled (ISEL) cells in cultures treated with EGCG at 6.25 to 200 μM increased dose-dependently. Furthermore, exposure to 6.25 to 50 μM EGCG for 24 hr led to a significant increase in caspase-3 activity compared to the control. These results suggest that EGCG induces apoptosis in NRK-49F cells.
- 2008-08-01
著者
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Miyamoto Yohei
Toxicology And Pharmacokinetics Laboratories Pharmaceutical Res. Laboratories Toray Industries Inc.
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Miyamoto Yohei
Toxicology And Pharmacokinetics Laboratories Pharmaceutical Research Laboratories Toray Industries I
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HAYLOR John
Sheffield Kidney Institute, Northern General Hospital NHS Trust, The University of Sheffield
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Sano Mariko
Toxicology and Pharmacokinetics Laboratories, Pharmaceutical Research Laboratories, Toray Industries
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El Nahas
Sheffield Kidney Institute, Northern General Hospital NHS Trust, The University of Sheffield
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El Nahas
Sheffield Kidney Institute Northern General Hospital Nhs Trust The University Of Sheffield
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Haylor John
Sheffield Kidney Inst. Northern General Hospital Nhs Trust The Univ. Of Sheffield
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Haylor John
Sheffield Kidney Institute Northern General Hospital Nhs Trust The University Of Sheffield
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Sano Mariko
Toxicology And Pharmacokinetics Laboratories Pharmaceutical Research Laboratories Toray Industries I
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