Topically Applied Diterpenoids from Egletes viscosa (Asteraceae) Attenuate the Dermal Inflammation in Mouse Ear Induced by Tetradecanoylphorbol 13-Acetate- and Oxazolone(Pharmacology)

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概要

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• The diterpene compounds, centipedic acid (CA) and 12-acetoxyhawtriwaic acid lactone (AHAL, tanabalin) isolated from the flower buds of Egletes viscosa LESS. (Asteraceae) were evaluated on acute and chronic models of mouse ear dermatitis. A single topical application of CA (0.125; 0.25 and 0.5mg/ear) or AHAL (0.125, 0.25, 0.5mg/ear) immediately before 12-O-tetradecanoylphorbol-13-acetate (TPA, 2.5 μg/ear) caused a dose-related significant inhibition of ear inflammatory edema and influx of polymorphonuclear cells, as evidenced by a decrease in ear thickness and reduced myeloperoxidase (MPO) activity and tumor necrosis factor-α (TNF-α) in ear tissue homogenates. The maximal obtained inhibition for both ear edema and neutrophil influx were almost similar to that of topically applied dexamethasone (0.05 mg/ear). The extent of inhibitions for the respective treatments of CA (0.5 mg/ear), AHAL (0.5 mg/ear), or dexamethasone (0.05 mg/ear) were in the order of 63%, 61% and 81% for the ear edema, and 90%, 95% and 95% for the neutrophil influx. Also, at similar doses, both diterpenes and dexamethasone effectively inhibited the delayed-type hypersensitivity reaction induced by repeated topical application of 1% oxazolone (OXA, 20 μl/ear), as evidenced by significant decreases in ear thickness and interferon-γ (INF-γ) levels in ear tissue. Histopathological analysis revealed a marked decrease in epidermal hyperplasia and neutrophil infiltration in animals pretreated with CA or AHAL, in a manner similar to dexamethasone. These data provide evidence for the anti-dermatitis effect of Egletes viscosa diterpenes, by mechanisms that involve a reduced neutrophil influx and decreased production of inflammatory cytokines, TNF-α and IFN-γ.

• 2008-08-01

著者

• Brito Gerly

  Department of Morphology, Federal University of Ceará

• Cunha Geanne

  Department Of Physiology And Pharmacology Faculty Of Medicine Federal University Of Ceara

• Santos Flavia

  Department Of Physiology And Pharmacology Post-graduate Programme In Medical Sciences Faculty Of Med

• Rao Vietla

  Department Of Physiology And Pharmacology Post-graduate Programme In Medical Sciences Faculty Of Med

• Rao Vietla

  Departament Of Physiology And Pharmacology Federal University Of Ceara (fm)

• Brito Gerly

  Department Of Morphology Federal University Of Ceara

• Silveira Edilberto

  Department Of Organic And Inorganic Chemistry Federal University Of Ceara

• Sousa Daniel

  Department Of Physiology And Pharmacology Faculty Of Medicine Federal University Of Ceara

• CALOU Iana

  Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara

• Calou Iana

  Department Of Physiology And Pharmacology Faculty Of Medicine Federal University Of Ceara

• Santos Flavia

  Departament Of Physiology And Pharmacology Federal University Of Ceara (fm)

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