血中δ-アミノレブリン酸の鉛曝露指標としての有用性

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The concentration of δ-aminolevulinic acid in blood (ALA-B) was determined using high performance liquid chromatography (HPLC). To improve the chromatographic separation and the recovery rate of ALA determination in blood, acetate buffer was used in the reaction mixture of fluorescence derivatization. The detection limit of ALA-B was ca. 2μg/l at signal to noise ratio of 5, and the analytical recovery was 102.0±4.10% (mean±SD), when 50μg/l of ALA was added to 7 blood samples (ALA-B levels: 6.5-103.0μg/l). ALA-B levels in control subjects (n=19) were 5.3±1.4μg/l (mean±SD) and those in 52 lead workers (blood lead levels (Pb-B): 2.4-86.2μg/dl) were 15.4±12.2μg/l (range: 3.1-137.3μg/l). Standard curve of ALA was linar over a wide range, at least up to 400μg/l. In the workers, the correlation coefficients of ALA-B vs. Pb-B and ALA-B vs. δ-aminolevulinic acid dehydratase activity (ALA-D) were higher than those of urinary concentration of ALA vs. Pb-B and that vs. ALA-D, especially in the moderate Pb-B level (less than 40μg/dl, n=35). Unless the urinary concentrations of ALA were not corrected, significant correlation could not be found between Pb-B and urinary ALA in the workers moderately exposed to lead, and the correlation coefficient between urinary ALA and ALA-D was -0.354, while the correlation coefficients of ALA-B vs. Pb-B and ALA-B vs. ALA-D were 0.739 and -0.746, respectively. The effect of lead on bone marrow is more directly indicated by ALA-B than urinary concentration of ALA. Hence, ALA-B is a useful parameter for biological monitoring of lead-exposed workers, especially in the wide range of Pb-B level.
社団法人日本産業衛生学会の論文
1993-03-20

血中δ-アミノレブリン酸の鉛曝露指標としての有用性

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