Study on the Interactions of Kaempferol and Quercetin with Intravenous Immunoglobulin by Fluorescence Quenching, Fourier Transformation Infrared Spectroscopy and Circular Dichroism Spectroscopy

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概要

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• The interactions of kaempferol and quercetin with intravenous immunoglobulin (IVIG) were studied in vitro by spectroscopic methods including fluorescence spectra, Fourier transformation infrared (FT-IR) spectra and circular dichroism (CD) spectra. The binding parameters for the reactions calculated according to the Sips equation suggested that the bindings of IVIG to kaempferol and quercetin were characterized by two binding sites with the average affinity constants K_0 at 1.032×10^4M^<-1> and 1.849×10^4M^<-1>, respectively. The binding of IVIG with quercetin is stronger than that of IVIG with kaempferol. They were of non-specific and weak drug-protein interactions. Docking was used to calculate the interaction modes between kaempferol and quercetin with IVIG. The secondary structural compositions of free IVIG and its kaempferol, quercetin complexes were calculated by the FT-IR difference spectra, self-deconvolution, second derivative resolution enhancement and the curve-fitting procedures of amide I band respectively, which are in good agreement with the analyses of CD spectra. The effect of 3'-OH substituent in quercetin is distinct between the interactions of IVIG with kaempferol and quercetin for the secondary structure of the protein. The observed spectral changes indicate a partial unfolding of the protein structure, but the typical β structural conformation of IVIG is still retentive in the presence of both drugs in aqueous solution. The average binding distances between the chromophores of IVIG with kaempferol (4.30 nm) and quercetin (4.35 nm) were obtained on the basis of the theory of Forster energy transfer. IVIG can serve as transport protein (carrier) for kaempferol and quercetin.

• 社団法人日本薬学会の論文
• 2008-04-01

著者

• Zheng Xu-Dong

  College of Chemistry and Chemical Engineering, Longdong University

• Hu Huai-Sheng

  College of Chemistry and Chemical Engineering, Longdong University

• Hu Huai-sheng

  Coll. Of Chemistry And Chemical Engineering Longdong Univ.

• Hu Huai-sheng

  College Of Chemistry And Chemical Engineering Longdong University

• Zheng Xu-dong

  Coll. Of Chemistry And Chemical Engineering Longdong Univ.

• Zheng Xu-dong

  College Of Chemistry And Chemical Engineering Longdong University

• YANG Zheng-Yin

  College of Chemistry and Chemical Engineering and State Key Laboratory of Applied Organic Chemistry,

• Yang Zheng-yin

  College Of Chemistry And Chemical Engineering And State Key Laboratory Of Applied Organic Chemistry

• Yang Zheng-yin

  College Of Chemistry And Chemical Engineering State Key Laboratory Of Applied Organic Chemistry Lanz

• LIU Yong-chun

  College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, La

• DU Juan

  College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, La

• YAO Xiao-jun

  College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, La

• LEI Rui-xia

  College of Chemistry and Chemical Engineering, Longdong University

• LIU Jian-ning

  College of Chemistry and Chemical Engineering, Longdong University

• LI Hong

  College of Chemistry and Chemical Engineering, Longdong University

• Lei Rui-xia

  College Of Chemistry And Chemical Engineering Longdong University

• Yao Xiao-jun

  College Of Chemistry And Chemical Engineering State Key Laboratory Of Applied Organic Chemistry Lanz

• Liu Jian-ning

  College Of Chemistry And Chemical Engineering Longdong University

• Liu Yong-chun

  College Of Chemistry And Chemical Engineering State Key Laboratory Of Applied Organic Chemistry Lanz

• Du Juan

  College Of Chemistry And Chemical Engineering State Key Laboratory Of Applied Organic Chemistry Lanz

• Du Juan

  College Of Biological Sciences And Biotechnology Beijing Forestry University

• Li Hong

  College Of Chemistry And Chemical Engineering Longdong University

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