Adenoviral Transfer of Rho Family Proteins to Lung Cancer Cells Ameliorates Cell Proliferation and Motility and Increases Apoptotic Change

スポンサーリンク

概要

• 論文の詳細を見る

• Lung cancer is still a very severe disease which has a low survival rate due to localinvasion and metastasis potentials in spite of many clinical challenges using anti-cancerdrugs. Rho family small GTPases play pivotal roles in cell invasion and metastasisduring carcinogenesis. In this study, we explored the inhibitory effect of adenoviralvector encoding dominant negative mutants of Rac, RhoA, and ROCK in humannon-small cell lung carcinoma cell lines (A549 and SQ5) and mouse carcinoma cell line(Lewis lung carcinoma, LLC). These cells showed high expression of Rac, Rho, andROCK, whereas only faint bands were detected in normal human lung epithelial cells,BET-1A. The efficiency of adenoviral vector transfer was stronger in A549 and SQ5cells than LLC cells. Dominant negative forms of RhoA (Rho-DN) and Rac (Rac-DN)decreased cell proliferation in WST-8 assay and increased the number of apoptotic cellsin both A549 and SQ5 cells by Hoechst 33258 and TUNEL staining. On the otherhand, DN form of ROCK (ROCK-DN) did not show any apparent changes comparedwith the other proteins. Transwell○!R chamber analysis showed that migration/invasionactivity was significantly suppressed by gene transfection both in A549 and SQ5 cellsand that ROCK-DN gene transfer required a higher multiplicity of infection to showeffects similar to Rho and Rac. Although the effect of gene therapy is cell-dependent,these data suggest that adenoviral gene transfer with Rho family small GTPases is onegood approach to lung cancer therapy.

• 神戸大学の論文

著者

• YOKOYAMA Mitsuhiro

  Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe Universit

• Rikitake Yoshiyuki

  Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe U

• Yokoyama Mitsuhiro

  Department Of Cardiovascular Medicine Awaji Prefectural Hospital

• Yokoyama Mitsuhiro

  Department Of Internal Medicine Division Of Cardiovascular Medicine Kobe University Graduate School

• Yokoyama Mitsuhiro

  Department Of Cardiology Kobe University Graduate School Of Medicine

• Yokoyama Mitsuhiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Yokoyama M

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Hirase Tetsuaki

  Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine

• Yokoyama M

  Department Of Cardiovascular Medicine Awaji Prefectural Hospital

• Yokoyama Mitsuhiro

  Kobe University Graduate School Of Medicine

• Yokoyama Mitsuhiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Midicine Kobe University

• Nishimura Yoshihiro

  Division of Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School o

• Yokoyama Mitsuhiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Hirase Tetsuaki

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Rikitake Yoshiyuki

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Shimada Temiko

  Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe Universit

• Nishimura Teruaki

  Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe Universit

• Shimada Temiko

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Yokoyama Mitsuhiro

  First Department Of Internal Medicine Kobe University

• Yokoyama Mitsuhiro

  Dept. Of Intern. Med. Div. I Kobe Univ. School Of Med.

• Hirase Tetsuaki

  Kobe University Graduate School of Medicine

• Yokoyama Mitsuhiro

  The First Department Of In Internal Medicine Kobe University School Of Medicine

• Rikitake Yoshiyuki

  The First Department Of Internal Medicine Kobe University School Of Medicine

• Nishimura Yoshihiro

  Division Of Respiratory Medicine Department Of Internal Medicine Kobe University Graduate School Of

• Yokoyama Mitsuhirc

  The First Department Of Internal Medicine Kobe University School Of Medicine

• Yokoyama Mitsuhiro

  Division Of Cardiovascular Medicine Kobe University Graduate School Of Medicine

• Yokoyama Mitsuhiro

  The 1st Department Of Internal Medicine Kobe University School Of Medicine

• Yokoyama Mitsuhiro

  The Department Of Internal Medicine (1st Division) Kobe University School Of Medicine

• Yokoyama Mitsuhiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Nakajima Takeo

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Nishimura Yoshihiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University

• Yokoyama Mitsuhiro

  Division Of Cardiovascular And Respiratory Medicine Department Of Internal Medicine Kobe University Graduate School Of Medical Science

関連論文

• Incremental Effects of Eicosapentaenoic Acid on Cardiovascular Events in Statin-Treated Patients With Coronary Artery Disease : Secondary Prevention Analysis From JELIS
• Trichostatin A, an Inhibitor of Histone Deacetylase, Inhibits Smooth Muscle Cell Proliferation via Induction of p21^
• Transforming Growth Factor-β Signaling Enhances Transdifferentiation of Macrophages into Smooth Muscle-Like Cells
• PJ-705 Lipid Peroxidation and Hemoglobin A1c are Major Risk Factors of Coronary Heart Disease (CHD). The Hidaka Cohort Study(Preventive medicine/Epidemiology/Education-6 (H) PJ118,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of
• 5 Remnant Lipoproteins, Strong Key Molecules to Atherogenesis in the Metabolic Syndrome(New Horizons in the Metabolic Syndrome:-Molecular Mechanisms and Therapies,Plenary Session 4 (PL4) (H),The 70th Anniversary Annual Scientific Meeting of the Japanese C
• Role of Transforming Growth Factor-β-Smad Signaling in Transdifferentiation of Macrophages into Smooth Muscle-like Cells (Vascular Smooth Muscle 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Impact of Increasing Diabetes on Coronary Artery Disease in the Past Decade
• OJ-517 Transforming Growth Factor-β_1 Enhances Transdifferentiation of Macrophages into Smooth Muscle-like Cells(Vascular Smooth Muscle 2 (H) : OJ63)(Oral Presentation (Japanese))
• OE-166 Transdifferentiation of monocytes/macrphages into smooth muscle-like cells(Restenosis after Angioplasty, Basic/Clinical 1 (IHD) : OE20)(Oral Presentation (English))
• A Case of Severe Hyperlipidemia Caused by Long-term Tube Feedings
• High Glucose Accelerates Monocyte Chemoattractant Protein-1 Production by Sequential Activation of p38 Mitogen-Activated Protein Kinase in Human Vascular Endothelial Cells
• 2 Major Outcomes and Safety of EPA Derived from the Japan EPA LIPID INTERVENTION STUDY (JELIS)(Late Breaking Clinical Trials in Japan,The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• Strategy for treating elderly Japanese with hypercholesterolemia
• Endothelial Lipase Gene Polymorphism is Associated With Acute Myocardial Infarction, Independently of High-Density Lipoprotein-Cholesterol Levels
• Presence of Reverse Fill-in on Stress-Reinjection 99m Tc-Tetrofosmin (TF) SPECT Prevents LV Remodeling in Patients after Myocardial Infarction with Reperfusion
• Abnormal Retention of 99mTc-Tetrofosmin (TF) Indicates Fatty-Acid Metabolic Dysfunction in Post-Reperfusion Myocardium
• Suppressive Effect of the Gly389 Allele of the β_1-Adrenergic Receptor Gene on the Occurrence of Ventricular Tachycardia in Dilated Cardiomyopathy
• β1-Adrenergic receptor gene polymorphism modulates cardiac sympathetic innervation in patients with dilated cardiomyopathy
• Correlation of Connexin43 Expression and Late Ventricular Potentials in Nonisehemic Dilated Cardiomyopathy
• Clinical Significance of T-Wave Alternans in Hypertrophic Cardiomyopathy
• Decreased Expression of Gap Junction Connexin43 Is Closely Associated with Life Threatening Ventricular Tachyarrhythmias and Late Potentials in Dilated Cardiomyopathy
• Usefulness of Beat-to-Beat QT Dispersion Fluctuation for Identifying Patients with Organic Heart Disease at Risk for Ventricular Arrhythmias
• Altered Expression of Gap Junctional Protein Connexin43 In Non-Ischemic Dilated Cardiomyopathy With Ventricular Tachycardia
• OE-233 Augmentation of Vascular Remodeling by Uncoupled eNOS in a Mouse Model of Diabetes Mellitus(Atherosclerosis, basic-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PE-289 Angiographical Analysis of Small Pulmonary Arteries in Pulmonary Hypertension by Synchrotron Radiation Microangiography(Pulmonary circulation-3 (H) PE49,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circula
• OE-205 Mouse Cardiac Cross-bridge Function Assessed in Vivo by An X-ray Diffraction : Effects of Adrenergic Beta-stimulation(Cardiac function, basic/clinical-1 (M) OE35,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japa
• OE-065 Local Overexpression of Toll-like Receptors at the Vessel Wall Induces Atherosclerotic Lesion : Synergism of Toll-like Receptor 2 and 4(Atherosclerosis, basic-1 (H) OE11,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of
• OE-009 The Possible Role of Oxidative Stress Caused by Uncoupled eNOS in Left Ventricular Remodeling after Myocardial Infarction in Rats(Chronic coronary heart disease/Remodeling-1 (IHD) OE2,Oral Presentation (English),The 70th Anniversary Annual Scientif
• Angiotensin II Type1 Receptor Blocker, Telmisartan Suppresses Superoxide Production and Atherosclerotic Lesion Formation in Apolipoprotein E-deficient Mice(Atherosclerosis, Basic 8 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Soci
• Increased GTP-cyclohydrolase I Expression but not Vitamin C Reversed the Accelerated Atherosclerotic Lesion Formation in Apolipoprotein E-deficient Mice Overexpressing eNOS(Endothelium/NO2 (H), The 69th Annual Scientific Meeting of the Japanese Circulatio
• PE-045 Overexpression of Endothelial Nitric Oxide Synthase Accelerates Vascular Remodeling in ApoE-deficient Mice(Endothelium/NO 1 (H) : PE8)(Poster Session (English))
• OJ-191 GTPCH I Overexpression Decreases Atherosclerotic Lesion Formation in Apolipoprotein E-Deficient/eNOS Transgenic Mice(Atherosclerosis, Basic 2 (IHD) : OJ22)(Oral Presentation (Japanese))
• Xenogenic Smooth Muscle Cell Immunization as a Vaccine Reduces Neointimal Formation in Balloon-Injured Rabbit Carotid Arteries
• Xenogenic Macrophage Immunization as a Vaccine Reduces Atherosclerosis in Apolipoprotein E Knockout Mice
• PE-095 Low Adiponectin Level is Associated with ACS Caused by Plaque Rupture Containing Lipid Necrotic Core(Acute coronary syndrome, basic/clinical-5, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PE-076 Delayed Neointimalization on Sirolimus-eluting Stents Observed by Optical Coherence Tomography(Restenosis basic/clinical-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Persistent Malapposition After Implantation of Sirolimus-Eluting Stent Into Intramural Coronary Hematoma : Optical Coherence Tomography Observations
• Impedance Cardiography for Cardiac Output Estimation : Reliability of Wrist-to-Ankle Electrode Configuration
• PJ-090 Evaluation of Sirolimus-eluting Stent Mal-apposition by Optical Coherence Tomography(Intravascular endoscopy/Intravascular ultrasound-6 (I) PJ15,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation So
• PE-481 Prognostic Importance of Increased Left Ventricular Myocardial Stiffness in Patients with Recurrent Diastolic Heart Failure(Heart failure, clinical-9 (M) PE81,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese C
• OJ-268 Optical Coherence Tomography Visualize Thin Neointima on Sirolimus-eluting Stent More Precisely than Intravascular Ultrasound at Six Month Follow-up(Intravascular endoscopy/Intravascular ultrasound-2 (I) OJ45,Oral Presentation (Japanese),The 70th A
• OJ-265 Plasma Adiponectin Levels are Associated with Plaque Component in Patients with Ischemic Heart Disease(Intravascular endoscopy/Intravascular ultrasound-2 (I) OJ45,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Ja
• OJ-129 Elevation of Plasma C-Reactive Protein is Associated with Histological Plaque Composition in Patients with Acute Coronary Syndromes(Acute coronary syndrome, basic/clinical-2 (IHD) OJ22,Oral Presentation (Japanese),The 70th Anniversary Annual Scient
• Effects of Human Atrial Natriuretic Peptide on Myocardial Performance and Energetics in Heart Failure due to Previous Myocardial Infarction(Cardiac Function, Basic/Clinical 4 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Usefulness of Cardiac Output Monitoring with a Novel Measure Using Peripheral Arterial Waveform Analysis in Patients with Heart Failure(Cardiac Function, Basic/Clinical 3 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Effectiveness of Catheter-based Trans-coronary Myocardial Hypothermia on Attenuating Myocardial Necrosis in Pigs with Acute Myocardial Infarction(Acute Myocardial Infarction, Clinical (Diagnosis/Treatment) 3 (IHD), The 69th Annual Scientific Meeting of th
• Enhancement of Ischemia-Induced Angiogenesis by eNOS Overexpression
• Rho/Rho Kinase pathway is involved in the regulation of NF-κB by TNF-α in cardiac myocytes
• Cardiac myocytes are recruited by bone marrow-derived cells in the intact murine heart
• Bezafibrate Improves Hypertension and Insulin Sensitivity in Humans
• Relationship Between Regional and Global Left Ventricular Systolic and Diastolic Function in Patients With Coronary Artery Disease Assessed by Strain Rate Imaging
• PE-277 Reduction of Functional Mitral Regurgitation is Associated with Improving Inferior Myocardial Function with Dobutamine Administration Assessed by Strain Rate Imaging(Valvular heart disease/Pericarditis/Cardiac tumor-2, The 71st Annual Scientific Me
• OE-365 Preoperative Systolic Strain Rate Predicts Postoperative Left Ventricular Systolic Function in Patients with Chronic Aortic Regurgitation(Valvular heart disease/Pericarditis/Cardiac tumor-1, The 71st Annual Scientific Meeting of the Japanese Circul
• Severe Regurgitation of the Left Atrioventricular Valve Associated With Corrected Transposition of the Great Arteries Treated by Valve Replacement
• PE-366 Quantitative Assessment of the Functional Mitral Regurgitation during Dobutamine Stress by Tissue Doppler Imaging(Echo/Doppler-8 (I) PE61,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• FRS-120 Improvement in Regional Myocardial Systolic Function Assessed by Transmural Myocardial Strain Profile after Surgical Correction for Chronic Aortic Regurgitation(Recent Advance in Echocardiography-2 (I) FRS24,Featured Research Session (English),The
• OJ-090 Impaired Regional Myocardial Strain Rate in Hypertrophic Cardiomyopathy Assessed by Tissue Doppler Echocardiography(Echo/Doppler 1 (I) : OJ11)(Oral Presentation (Japanese))
• PE-234 Serum Beta2-microglobulin Levels Strongly Predict the Prognosis of Heart Failure in the Very Elderly Patients(Heart failure, clinical-07, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Attenuation of Biological Compensation of Natriuretic Peptide System With Aging : Utility and Limitation of Brain Natriuretic Peptide in the Elderly
• Dobutamine stress technetium-99m tetrofosmin (Tc-TF) gated SPECT for predicting response of beta-blocker therapy in patients with idiopathic dilated cardiomyopathy (DCM)
• The relationship between serotonin-2A (5HT2A) receptor gene polymorphisms and 5HT-induced platelet aggregation
• Relation between myocardial interstitial fibrosis and autonomic nervous function in the patients with idiopathic dilated cardiomyopathy
• Overexpression of Endothelial Nitric Oxide Synthase Attenuates Cardiac Hypertrophy Induced by Chronic Isoproterenol Infusion
• OE-163 Atherosclerotic Plaque Imaging using Phase-Contrast X-ray Computed Tomography : Seeking for Clinical Application(CT/DSA(01)(I),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• PE-320 Atherosclerotic Plaque Imaging by Interferometric Phase-Contrast X-ray Computed Tomography(CT/DSA-07, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Echocardiographic Assessment of Right Ventricular Obstruction in Hypertrophic Cardiomyopathy
• 2 The Great Hanshin-Awaji Earthquake and Coronary Artery Disease(Cardiovascular Crisis in National Disasters,Topic 2 (TP2) (H),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• Endothelial Lipase is Increased by Inflammation and Promotes LDL Uptake in Macrophages
• Targeted Disruption and Overexpression of Endothelial Cell-Derived Lipase Modulates Plasma HDL and LDL Cholesterol Levels
• Expression of G2A, a Receptor for Lysophosphatidylcholine, by Macrophages in Murine, Rabbit and Human Atherosclerotic Plaques
• In Vivo Angiographic Detection of Vascular Lesions in Apolipoprotein E-Knockout Mice Using a Synchrotron Radiation Microangiography System
• A HMG-CoA Reductase Inhibitor Reduces Hypertensive Nephrosclerosis In Stroke Prone-SHR
• Role of endothelial nitric oxide in sphingosine 1-phosphate-induced angiogenesis
• A HMG-CoA Reductase Inhibitor Reduces Stroke Events and Mortality In Stroke-Prone Spontaneous Hypertensive Rats
• Supplement of tetrahydrobiopterin inhibits atherosclerosis in apoE-deficient mice
• In Vivo Mouse Coronary Angiography Using Synchrotron Radiation Microangiography
• Overexpression of endothelial nitric oxide synthase accelerates atherosclerotic lesion development in apoE-deficient mice
• OE-319 Nectin-2 is Required to Prevent Cardiac Fibrosis and Dysfunction in Response to Chronic Pressure Overload(Molecular biology, myocardium-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• OE-327 Why can myoblast transplantation combined with cardiotrophin-1-gene transfection alleviate the transition to heart failure in Dahl rats?(Gene Therapy 2 (IHD) : OE41)(Oral Presentation (English))
• Glycogen Synthase Kinase-3β is Involved in the Process of Myocardial Hypertrophy Stimulated by Insulin-Like Growth Factor-1
• Transplantation of Cardiotrophin-1-Expressing Myoblasts to Myocardium Prevents the Transition from Compensatory Hypertrophy to Congestive Heart Failure in Dahl Salt-Sensitive Rats
• Ral GDP Dissociation Stimulator (Ral-GDS) and Ral GTPase Are Involved in Cardiac Hypertrophy
• LIF Regulates 4E-BP1 Phosphorylation via PI 3-kinase-Akt Pathway In Myocardial Cells
• Cardiac Myocytes are Recruited by Bone Marrow-Derived Cells in Intact Murine Heart
• Identification of Functional Block Line in Atrial Flutter Using Three-Dimensional Intracardiac Echocardiography
• PE-347 Determinants of Plaque Progression in Patients with Stable Angina : Serial Intravascular Ultrasound Virtual Histology Study(Intravascular endoscopy/Intravascular ultrasound-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• OJ-133 Impact of Reactive Oxygen Species on Plaque Formation and Vessel Remodeling : Comparison of Histochemical Characteristics and IVUS Finding(Atherosclerosis, Clinical 2 (IHD) : OJ16)(Oral Presentation (Japanese))
• Interaction of Oxidative Stress and Inflammatory Response in Coronary Plaque Stability : Important Role of C Reactive Protein
• Multivariate Analysis of Hemodynamic Parameters for Increase in Plasma BNP in Systolic and Diastolic Heart Failure ; Importance of Myocardial Stiffness(Heart Failure, Clinical 13 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Close Correlation of Endothelial Function between Coronary and Brachial Circulation in Patients with Idiopathic Dilated Cardiomyopathy
• Antioxidant Agent, Glutathione Improves Contractile Response to Dobutamine and Mechanical Efficiency in Patients with Reduced Left Ventricular Function
• Depletion of Antioxidants Is Associated with No-Reflow Phenomenon in Acute Myocardial Infarction
• Acute Effects of Angiotensin II Receptor Blocker on Ventricular Repolarization Alternans in Chronic Heart Failure
• 2 Identification of the Right ventricular Pacing Site for Cardiac Resynchronization Therapy (CRT) Guided by Electroanatomical Mapping (CARTO)(Identification Strategy and Treatment Approach of the Non-responder Group in Patients Withcardiac Resynchronizati
• 1 Results of Valve-Sparing Aortic Root Replacement for Annuloaortic Ectasia and Moderate to Severe Aortic Regurgitation(Aortic Valve-Sparing Operations In Patients With Aortic Root Aneurysms, The 71st Annual Scientific Meeting of the Japanese Circulation
• Transthoracic Doppler Echocardiographical Assessment of Coronary Flow Velocity Characteristics in Patient with Chronic Aortic Regurgitation
• Contribution of inner and outer halves to transmural wall thickening in the hyperdynamic state using tissue Doppler tracking technique
• Role of Angiotensin and the Clock System in the Circadian Regulation of Plasminogen Activator Inhibitor-1
• Inhibition of Endogenous Nitric Oxide Synthase Augments Contractile Response to Adenylyl Cyclase Stimulation Without Altering Mechanical Efficiency in Patients With Idiopathic Dilated Cardiomyopathy
• PJ-450 Usefulness of Assessing Myocardial Washout of ^Tc-tetrofosmin for Prediction of Prognosis in Patients with Cardiac Sarcoidosis(Cardiomyopathy, basic/clinical-7, The 71st Annual Scientific Meeting of the Japanese Circulation Society)

もっと見る 閉じる

スポンサーリンク