Cardiac Myocytes are Recruited by Bone Marrow-Derived Cells in Intact Murine Heart

概要

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It is generally believed that the cardiac myocytes withdraw from the cell cycleshortly after birth and thereafter any loss of myocardial tissue cannot be repaired.However, recent reports indicate that cardiac myocytes can be regenerated by stemcells derived from bone marrow in the damaged hearts. In this study, we investigatedwhether bone marrow-derived cells can differentiate into cardiac myocytes in the intacthearts. We performed bone marrow transplantation from syngenic male mice to femalec57/B6 mice. In female mices hearts, the presence of cells from male mice wasexamined by FISH method that detects Y chromosome. Using the same samples, we alsoperformed immunohistochemical staining with muscle specific antibodies. In the heartsections of female mice, there were some cells that were considered as differentiatedmyocytes derived from male bone marrow (0.01~0.09% of total myocytes) and theproportion of the cells increased as the period after bone marrow transplantationbecame longer (3 months after vs. 8 months after). These results suggest that, not onlyin the damaged heart but also in the intact heart, a portion of cardiac myocytes isrecruited by bone marrow-derived cells.
神戸大学の論文