Hyperosmolarity Attenuates the Contraction of Rat Trachea Through the Inhibition of Phosphatidylinositol Response
スポンサーリンク
概要
- 論文の詳細を見る
Although hyperosmolarity associated with diabetes is known to attenuate contractile response of airway smooth muscle, intracellular mechanisms involved are not fully understood. We examined the effects of hyperosmolarity on carbachol (CCh)- and aluminum fluoride (AF)-induced contractile and phosphatidylinositol (PI) responses of rat trachea. In vitro measurements of isometric tension and [3H] inositol monophosphate (IP1) formed were conducted by using rat tracheal rings and slices. Hyperosmolarity solutions of 350, 450 and 600 mOsm were made with dissolving glucose in Krebs-Henseleit (K-H) solution. Hyperosmolarity attenuated dose-dependently CCh-induced contraction of rat trachea (1.86 ± 0.13 g at 300 mOsm, 1.85 ± 0.16 g at 350 mOsm, 1.37 ± 0.07 g at 450 mOsm and 0.50 ± 0.04 g at 600 mOsm, respectively), and also attenuated CCh- induced IP1 accumulation (5.77 ± 0.33 Bq at 300 mOsm, 3.38 ± 0.26 Bq at 350 mOsm, 2.08 ± 0.30 Bq at 450 mOsm and 1.71 ± 0.40 Bq at 600 mOsm, respectively), and AF-induced IP1 accumulation (3.93 ± 0.22 Bq at 300 mOsm, 1.63 ± 0.14 Bq at 450 mOsm and 1.02 ± 0.14 Bq at 600 mOsm, respectively). The results suggest that hyperosmolarity would inhibit G-protein-coupled phospholipase C, resulting in attenuation of CCh-induced airway smooth muscle contraction.
- 長崎大学の論文
著者
-
Saito Masataka
Department Of Anesthesiology Nagasaki University School Of Medicine
-
Saito Masataka.
Departments Of Anesthesiology Nagasaki University School Of Medicine
関連論文
- Effects of sevoflurane compared with those of isoflurane on arterial oxygenation and hemodynamics during one-lung ventilation
- Effects of propofol and ketamine on ATP-induced contraction of the rat trachea
- Effects of ketamine on neostigmine-induced contractile and phosphatidylinositol responses of the rat trachea
- Effects of vasopressors on contractile and phosphatidylinositol responses of rat trachea
- Effects of ATP-sensitive potassium channel openers on the contractile and phosphatidylinositol responses of the rat trachea
- Scutellariae Radix augments ulceration but attenuates proinflammatory cytokine and chemokine gene induction in the small intestine during indomethacin-induced enteropathy in mice
- Bofutsushosan, a traditional Chinese formulation, inhibits pancreatic lipase activity in vitro and suppresses the elevation of plasma triacylglycerols after oral administration of lipid emulsion
- Hyperosmolarity Attenuates the Contraction of Rat Trachea Through the Inhibition of Phosphatidylinositol Response
- Genotype-phenotype correlations in six Japanese patients with recessive dystrophic epidermolysis bullosa with the recurrent p.Glu2857X mutation
- Differential Effects of Selective Cyclooxygenase (COX)-1 and COX-2 Inhibitors on Anorexic Response and Prostaglandin Generation in Various Tissues Induced by Zymosan(Biochemistry)