木本および草本植物内の緑色組織に含まれる色素の同定と光合成産物の枝・茎内分布 : 基礎技術の確立とその応用

概要

論文の詳細を見る
To acquire firm knowledge on the carbon fixation in woody and herbaceous plants, our attention was focused on the greenish tissue under the bark, subcorticular chrolenchyma. Greenish tissues of 28 of woody plants, such as Prunus and Pinus, and eight of herbaceous ones, were investigated using thin layer chromatography, absorption spectroscopy, spectrofluorometry, far-red (FR) imaging technique and iodine-starch reaction. Chromatogram of the greenish-tissue extract revealed at least four distinct bands which were identified as those originated from Chl a, Chl b, carotene, and pheophytin by comparing to those from leaf extract. The band assignment was also confirmed on the basis of absorption spectra. Fluorescence from greenish tissue induced by the blue light had two λ_<max> at around 685nm and 740nm. Distribution of the greenish tissue in the stem, as well as fluorescence, has changed depending on seasons of the year and plant's age. Intracellular distribution of fluorescent particles was easily observed but that of scattered greenish ones was rather difficult. Both of starch and green pigments seemed to coexist in the ray, but their coexistence was not necessarily proved in the other tissues. These results suggest that greenish tissue is contained chlorophyll within stems in any species and that chlorenchymal activity might contribute to the overall carbon-fixation in plants.
石巻専修大学の論文