Purification and Properties of Dye-linked Aldehyde Dehydrogenase in Rhodopseudomonas acidophila M402(Biological Chemistry)

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概要

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• Rhodopseudomonas acidophila M402 grown under aerobic-dark condition shows two bands of dye-linked aldehyde dehydrogenase activity by polyacrylamide gel electrophoresis. The slower-migrating band coincided with the dye-linked aromatic alcohol dehydrogenase that was active on aromatic and aliphatic alcohols and aldehydes. The faster-migrating band was a new dye-linked aldehyde dehydrogenase. The latter enzyme was purified 125-fold by ultracentrifugation and column chromatographies on DEAE-cellulose, Bio-Gel HTP, and Sepharose CL-6B. The enzyme has a relative molecular mass of 70,000 daltons, and a subunit size of 35,000 dalton indicates the dehydrogenase has a dimeric structure. The isoelectric point was pH 4.74. NAD^+ and NADP^+ do not act as electron acceptors. The enzyme was active specifically on straight chain aldehydes (C3-C10) rather than on benzaldehyde and its substitutes. Aromatic and aliphatic alcohols were inert for this enzyme. The Michaelis constant (mM) were 1.6, 0.6, 0.9, 3.6, 0.5, 0.3, 0.1, 0.9, 0.6, 0.3, and 0.1 for benzaldehyde, m-hydroxybenzaldehyde, m-anisaldehyde, vanillin, propionaldehyde, butyraldehyde, hexaldehyde, heptaldehyde, octaldehyde, nonaldehyde, and decaldehyde, respectively.

• 社団法人日本農芸化学会の論文
• 1991-04-23

著者

• Oikawa Tadao

  Institute Of Applied Biochemistry The University Of Tsukuba:(present Office)institute For Chemical R

• YAMANAKA Kei

  Institute of Applied Biochemistry, The University of Tsukuba

• IINO Hideaki

  Institute of Applied Biochemistry, The University of Tsukuba

• Iino Hideaki

  Institute Of Applied Biochemistry The University Of Tsukuba

• Yamanaka Kei

  Institute Of Applied Biochemistry The University Of Tsukuba:(present Office)department Of Applied Ch

関連論文

• Purification and Properties of Dye-linked Aldehyde Dehydrogenase in Rhodopseudomonas acidophila M402(Biological Chemistry)
• Capture of Pine-wilt Nematodes by Arthrobotrys ellipsospora Y4007. Mucin-specific Hemagglutinin and Its Role in the Capture(Microbiology & Fermentation Industry)
• Identification and Characterization of a Nicotinamide Adenine Dinucleotide-Dependent p-Hydroxybenzyl Alcohol Dehydrogenase from Rhodopseudomonasacidophila M402
• Comparison of TwoDye-linked Alcohol Dehydrogenases of Rhodopseudomonas acidophila: Their Substrate Specificity and Behavior toward Oxygen
• A New Dye-Linked Alcohol Dehydrogenase (Vanillyl Alcohol Dehydrogenase) from Rhodopseudomonas acidophila M402 Purification, Identification of Reaction Product and Substrate Specificity
• Multiple Forms of Inductive and Constitutive Acyl-CoA Synthetases in Rhodopseudomonas palustris No. 82
• Isolation and Characterization of a Methanol-utilizing Phototrophic Bacterium, Rhodopseudomonas acidophila M402 and Its Growth on Vanillin Derivatives
• Butyrate Activation in Rhodopseudomonas palustris No. 82
• Occurrence of Dehydrogenases for the Metabolism of Vanillyl Alcohol in Rhodopseudomonas acidophila M402

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