Capture of Pine-wilt Nematodes by Arthrobotrys ellipsospora Y4007. Mucin-specific Hemagglutinin and Its Role in the Capture(Microbiology & Fermentation Industry)

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The host-predator relationship between the pine-wilt nematode, Bursaphelenchus xylophilus, and the nematophagous fungus, Arthrobotrys ellipsospora Y4007, was investigated. An adhesive trapping knob comprises the trapping organ of this fungus. On scanning electron microscopy, ring structures and net structures were not observed at either the initial stage of trapping and or after prolonged contact of nematodes with hyphae for 5 days under the experimental conditions. This fungus produced hemagglutinating substance(s), which were detected in mycelia and in the cultural medium. This Arthrobotrys hemagglutinin was purified from the cultural filtrate and its specificity toward sugars was assayed. Out of 20 sugars (mono-, di- and oligosaccharides, sugar alcohols and N-acetylhexosamines) tested, only bovine submaxillary mucin inhibited the hemagglutination effectively. A partially purified non-proteinous fraction of the nematode-extract showed high affinity to the Arthrobotrys ellipsospora hemagglutinin and inhibited the hemagglutination completely at 0.0004%. Arthrobotrys hyphae treated with bovine submaxillary mucin lost their capture ability and over 80% of the added nematodes were recovered from the fungal mat by the Baermann funnel technique.
社団法人日本農芸化学会の論文
1988-03-23