Comparison of TwoDye-linked Alcohol Dehydrogenases of Rhodopseudomonas acidophila: Their Substrate Specificity and Behavior toward Oxygen

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概要

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• Two dye-linked alcohol dehydrogenases (EC 1.1.99.8) were purified from extracts of Rhodopseudomonas acidophila 10050 grown anaerobically on methanol and extracts of R. acidophila M402grown aerobically on vanillyl alcohol. Both dehydrogenases have the same name based on the rules of enzyme nomenclature, and have similar properties in coupling to phenazine methosulfate and requiring ammonia as an activator, but differences between the two enzymes could be pointed out in many aspects, especially as to their substrate specificities. The former enzymeis active on methanol, whereas the latter is enzyme is completely inert on methanol. While the former enzyme does not act on vanillyl alcohol, the latter enzyme shows high affinity for vanillyl alcohol and some aromati alcohols. Another evident difference is the behavior of the former enzyme toward oxygen. The methanol-induced enzymewas sensitive to oxygen. The enzyme lost its activity on exposure to oxygen, but the activity could be recovered by passing nitrogen gas over it for a few minutes. Therefore, these inactivation and reactivation changes of the enzymewere reversible. This implied a change of affinity constants for substrate alcohols on nitrogen treatment. Apparent Km values for methanol, ethanol, n-propanol and n-butanol were lowered to one-tenth the original values with nitrogen gas treatment, while maximumreaction velocities on these alcohols remained unchanged after nitrogen treatment. We propose that the methanol-induced enzyme should be called an anoxygenic dye-linked alcohol dehydrogenase or dye-linked aliphatic alcohol dehydrogenase. The latter enzyme which is induced by vanillyl alcohol is better named a dye-linked alcohol (non-specific) dehydrogenase.

著者

• YAMANAKA Kei

  Institute of Applied Biochemistry, The University of Tsukuba

• Yamanaka Kei

  Institute Of Applied Biochemistry The University Of Tsukuba:(present Office)department Of Applied Ch

• Minoshima Ryoichi

  Graduate School of Environmental Sciences, The University of Tsukuba

関連論文

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• Identification and Characterization of a Nicotinamide Adenine Dinucleotide-Dependent p-Hydroxybenzyl Alcohol Dehydrogenase from Rhodopseudomonasacidophila M402
• Comparison of TwoDye-linked Alcohol Dehydrogenases of Rhodopseudomonas acidophila: Their Substrate Specificity and Behavior toward Oxygen
• A New Dye-Linked Alcohol Dehydrogenase (Vanillyl Alcohol Dehydrogenase) from Rhodopseudomonas acidophila M402 Purification, Identification of Reaction Product and Substrate Specificity
• Multiple Forms of Inductive and Constitutive Acyl-CoA Synthetases in Rhodopseudomonas palustris No. 82
• Isolation and Characterization of a Methanol-utilizing Phototrophic Bacterium, Rhodopseudomonas acidophila M402 and Its Growth on Vanillin Derivatives
• Butyrate Activation in Rhodopseudomonas palustris No. 82
• Occurrence of Dehydrogenases for the Metabolism of Vanillyl Alcohol in Rhodopseudomonas acidophila M402

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