The Synthesis of New Xylosyloligosaccharides by Transxylosylation with Aspergillus niger β-Xylosidase(Microbiology & Fermentation Industry)

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概要

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• The synthesis of xylosyloligosaccharides from β-(1→4)-xylobiose in the presence of D-mannose by transxylosylation with β-xylosidase from Aspergillus nlger IFO 6662 was studied. At first, the transxylosylation products were separated by charcoal column chromatography in 5% ethanol elution into three peaks, P-1, P-2, and P-3. They were further separated on thin layer chromatography, and their three products, saccharides O-4, O-5, and O-N were purified by preparative paper chromatography. Saccharides O-4 and O-5 were identified as O-β-D-xylopyranosyl-(1→4)-D-mannopyranose and 0-β-D-xylopyranosyl-(1→6)-D-mannopyranose, respectively, by measurement of the degree of polymerization, specific rotation, acid hydrolysis, and methylation analysis. By ^1H-NMR spectroscopy in addition to these analyses, saccharide O-N was identified as O-β-D-xylopyranosyl-(1→1')-β-D-xylopyranose, a new nonreducing xylobiose.

• 社団法人日本農芸化学会の論文
• 1991-03-23

著者

• SHINOYAMA Hirofumi

  Institute of Applied Biochemistry, University of Tsukuba

• KIZAWA Hideki

  Institute of Applied Biochemistry, University of Tsukuba

• Kizawa Hideki

  Institute Of Applied Biochemistry University Of Tsukuba:(present Office)intergrated Technology Labor

• YASU Tsuneo

  Institute of Applied Biochemistry, University of Tsukuba

• Yasu Tsuneo

  Institute Of Applied Biochemistry University Of Tsukuba

• Shinoyama Hirofumi

  Institute Of Applied Biochemistry University Of Tsukuba:(present Office)department Of Agricultual Ch

• Shinoyama Hirofumi

  Institute Of Applied Biochemistry University Of Tsukuba

関連論文

• The Condensation Reaction of Aspergillus niger Crude β-Xylosidase Using Xylose
• Enzymatic Synthesis of Alkyl β-Xylosides from Xylobiose by Application of the Transxylosyl Reaction of Aspergillus niger β-Xylosidase (Biological Chemistry)
• A Novel Nonreducing Disaccharide, O-β-D-Xylopyranosyl-(1→1')-β-D-Xylopyranose, Produced from Xylobiose by Transxylosylation with Aspergillus niger β-Xylosidase(Biological Chemistry)
• Superiority of Aspergillus niger β-Xylosidase for the Enzymatic Synthesis of Alkyl β-xylosides in the Presence of a Variety of Alcohols (Biological Chemistry)
• 414 Screening of B-Mannosidase Suitable for the Enzymatic Synthesis of Alkyl-β-Mannosides.
• The Synthesis of New Xylosyloligosaccharides by Transxylosylation with Aspergillus niger β-Xylosidase(Microbiology & Fermentation Industry)

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