Binding-site Analysis of the Ether Linkages between Lignin and Hemicelluloses in Lignin-Carbohydrate Complexes by DDQ-Oxidation(Analytical Chemistry)

概要

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Lignin-carbohydrate complexes (LCC ; nor-C-1-M, com-C-1-A) isolated from normal and compression woods of Pinus densiflora were hydrolyzed with two types of cellulase preparations, and the hydrolyzates formed were fractionated by adsorption chromatography on polyvinyl gel into water-soluble materials and LCC fragments. To elucidate the binding sites between the lignin and carbohydrate, the cellulase-degraded LCC fragments were subjected to acetylation, and then oxidation with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which was confirmed to oxidatively cleave the benzyl ether linkages between the lignin and carbohydrate. The DDQ-oxidized fraction was then methylated by the method of Prelim, hydrolyzed, reduced and acetylated. A GC-MS analysis of the methylated sugar revealed that alditol acetates from 6-0-methyl mannose, 6-0-methyl galactose, 6-0-methyl glucose and a small amount of their 2-0- or 3-0-methyl isomers existed in both methylated fractions. 2-0-Methyl xylose and 3-0-methyl xylose were also identified in the fraction from the acidic LCC (com-C-1-A). These results led to the conclusion that acetylglucomannan and β-1,4-galactan were preferably bound to the lignin at C-6 position of the hexoses, and that arabinoglucuronoxylan did likewise at the C-2 and C-3 positions of xylose units.
社団法人日本農芸化学会の論文
1989-08-23