Purification and Some Properties of a Castor-oil-hydrolyzing Lipase from Pseudomonas sp.(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• To obtain a lipase which effectively hydrolyzes castor oil, bacteria were isolated from 500 soil samples. The best strain was examined; its microbiological characteristics suggested that it belongs to the genus Pseudomonas. A lipase from this strain was purified by ammonium sulfate fractionation and chromatographies on DEAE-cellulose and DEAE-Toyopearl 650 M. The enzyme was purified about 400-fold with a yield of 13%. The purified enzyme was electrophoretically homogeneous and its molecular weight was 30,000. The optimum pH and temperature for the hydrolysis of olive oil emulsion were 7.0 and 60℃. The enzyme was stable up to 35℃ at pH 7.0 for 30 min and also stable from pH 9.0 to 10.0 at 4℃ for 22 hr. The activity was inhibited by Fe^<3+>, Hg^<2+>, pCMB, and anionic surfactants, and enhanced by nonionic surfactants and bile salts. The enzyme efficiently hydrolyzed castor oil.

• 社団法人日本農芸化学会の論文
• 1988-12-23

著者

• Fujiwara Nobuaki

  Osaka Prefectural Industrial Research Institute

• YAMAMOTO KAZUHIKO

  Osaka Prefectural Industrial Technology Research Institute

関連論文

• Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
• Kinetics and Mechanism of Enzymatic Hydrolysis of Gelatin Layers of X-Ray Film and Release of Silver Particles
• Utilization of a Thermostable Alkaline Protease from an Alkalophilic Thermophile for the Recovery of Silver from Used X-Ray Film
• Purification and Some Properties of a Castor-oil-hydrolyzing Lipase from Pseudomonas sp.(Biological Chemistry)
• The Hydrolysis of Castor Oil Using a Lipase from Pseudomonas sp. f-B-24 : Positional and Substrate Specificity of the Enzyme and Optimum Reaction Conditions
• Production of Alkaline Protease in a Low-Cost Medium by Alkalophilic Bacilus sp. and Properties of the Enzyme

スポンサーリンク