Production of Alkaline Protease in a Low-Cost Medium by Alkalophilic Bacilus sp. and Properties of the Enzyme

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概要

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• The main object of this research was to obtain a large amount of alkaline protease in a low-cost medium, for which purpose Bacillus sp. B21-2 was isolated from soil. The enzyme production by this strain reached the maximum level of 15,000 u/ml in a short cultivation time of 24-28 h in alkaline medium consisting of cheap materials such as soybean meal and bonito extract. The optimum pH and temperature for activity of the purified enzyme were 11.5 and 60℃ respectively. The enzyme was stable up to 50℃ but inactivated after 10 min at 60℃. The enzyme activity was completely inhibited by diisopropylfluorophosphate.

• 公益社団法人日本生物工学会の論文
• 1987-06-25

著者

• Fujiwara Nobuaki

  Osaka Prefectural Industrial Research Institute

• YAMAMOTO KAZUHIKO

  Osaka Prefectural Industrial Technology Research Institute

• Yamamoto K

  Technology Research Institute Of Osaka Prefecture

関連論文

• Rational Design for Stabilization and Optimum pH Shift of Serine Protease AprN
• Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Protease in Baciilus subtilis
• Kinetics and Mechanism of Enzymatic Hydrolysis of Gelatin Layers of X-Ray Film and Release of Silver Particles
• Utilization of a Thermostable Alkaline Protease from an Alkalophilic Thermophile for the Recovery of Silver from Used X-Ray Film
• Purification and Some Properties of a Castor-oil-hydrolyzing Lipase from Pseudomonas sp.(Biological Chemistry)
• The Hydrolysis of Castor Oil Using a Lipase from Pseudomonas sp. f-B-24 : Positional and Substrate Specificity of the Enzyme and Optimum Reaction Conditions
• Production of Alkaline Protease in a Low-Cost Medium by Alkalophilic Bacilus sp. and Properties of the Enzyme

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