Isolation and Properties of a New β-Galactosidase Inhibitor, Galactostatin, from Streptomyces lydicus(Microbiology & Fermentation Industry)

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A new β-galactosidase inhibitor was found with a newly developed rapid and practical screening method for β-galactosidase inhibitors. Several strains of Actinomycetes that produced β-galactosidase inhibitors were isolated from soil samples using this method, and Streptomyces lydicus PA-5726 was found to produce a high concentration of new inhibitor in the culture medium. Improvement of the medium led to 20〜25-fold augmentation (45 to 900〜1,100μg/ml) of the production. This new inhibitor, which was named galactostatin, was isolated by adsorption on Dowex-50W×8 (H^+), crystallization from the bisulfite adduct, development on Dowex-2×8 (OH^-) and precipitation with EtOH. Galactostatin displayed strong inhibitory activity toward several β-galactosidases in acidic and neutral media but low acute toxicity in mice (LD_<50> 1016mg/kg, i.v.).
社団法人日本農芸化学会の論文
1988-01-23