Inhibition of β-Galactosidase by Galactostatin, Galactostatin-lactam, and 1-Deoxygalactostatin(Biological Chemistry)

概要

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Galactostain obtained from the fermentation broth of Streplomyces lydicus PA-5726 strongly inhibited β-galactosidase. Its derivatives, galactostatin-lactam and 1-dexygalactostain, were also inhibitors. Galactostatin and 1-deoxygalactostatin were fully competitive inhibitors with high affinities for Penicillium multicolor β-galactosidase, and their Ki values were 4.0×10^<-9> and 3.3×10^<-8>M at pH 6.0, respectively, using ONPG as substrate. In their presence, the steady-state velocities of the enzyme were reached in a matter of minutes. Galactostatin-lactam, in contrast, showed no detectable lag time on interaction with the enzyme, and the type of inhibition was also competitive with a Ki value of 1.3×10^<-5>M. These three inhibitors bound to the enzyme in the same molar ratio (1:1).
社団法人日本農芸化学会の論文
1988-07-23