Molecular Cloning of Dmrt1 and Its Expression in the Gonad of Xenopus(Reproductive Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Sex is determined by various transcription factors in vertebrates. SRY on the Y chromosome in mammals has been isolate as the sex-determining gene. In birds, Dmrt1 on the Z chromosome of chickens is expressed in the indifferent gonad before sex determination. In amphibians, the sex-determining gene is not known yet. To examine whether Dmrt1 is expressed during sex determination in Xenopus leavis, we first isolated the cDNA, and examined its expression in gonadal development by RT-PCR analysis. The expression of Xenopus Dmrt1 (xDmrt1) was observed exclusively in the testis amosis. The expression was much higher in the testis than in the ovary. This was a pattern different from that in human, mouse, and fish such as tilapia and rainbow trout. In addition, the Dmrt1 mRNA ws first detected in the gonad/mesonephros complex of tadpoles at stage 53, at which time the gonads were still indifferent. Taken together, the results suggest that Dmrt1 may be involved in gonadal differentiation of Xenopus.
- 社団法人日本動物学会の論文
- 2005-06-25
著者
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NAKAMURA Masahisa
Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University
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Nakamura Masahisa
Waseda Univ. Tokyo Jpn
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Oshima Yuki
Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University
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Osawa Noboru
Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University
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Nakamura M
Hiroshima Univ. Hiroshima Jpn
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Nakamura Masahisa
Department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University
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Nakamura Masahisa
Department Of Biology Faculty If Education And Integrated Arts And Sciences Waseda University
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Oshima Yuki
Department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University
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Osawa Noboru
Department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University
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Oshima Yuki
Waseda Univ. Tokyo Jpn
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Oshima Yuki
Department of Applied Quantum Physics and Nuclear Engineering, Kyushu University, Fukuoka 819-0395, Japan
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