Vitellogenin-immunohistochemistry in the Liver and the Testis of the Medaka, Oryzias latipes, Exposed to 17β-estradiol and ρ-nonylphenol(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
Vitellogenin (VTG) produced in male fish has been used for a biomarker to study endocrine disrupters. However, the characteristics of VTG produced in male fish have not been studied well. In this study, we investigated the localization of VTG in the liver and the testis of male medaka (Oryzias latipes) treated with 17β-estradiol (E2) and ρ-nonylphenol (NP). The male fish were exposed to 1 μg/L E2 and 500 μg/L NP for 1-12 days. Control groups were kept in water including only vehicle. The frozen sections of the liver and the testis were stained with immunohistochemical methods using an antiserum against medaka VTG as the first antibody. In the E2 and NP treated liver, the hepatocytes showed immunoreactivity. In particular, the cytoplasm close to the cell membrane surrounding the sinusoids was strongly immunopositive. In the testis of both treatments, the interstitial tissues and the cells (spermatocytes) in the seminiferous tubules were immunopositive. The concentration of VTG became gradually higher in both tissues with longer treatments. These results suggest that germ cells in the testis treated with E2 and NP are able to incorporate and accumulate VTG.
- 社団法人日本動物学会の論文
- 2005-04-25
著者
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TAMOTSU Satoshi
Graduate School of Humanities and Science, Nara Women's University
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OISHI Tadashi
Graduate School of Humanities and Science, Nara Women's University
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KOBAYASHI Kayo
Graduate School of Humanities and Sciences, Nara Women's University
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Kobayashi Keiko
Graduate School Of Science And Technology Niigata University
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Oishi T
Department Of Chemistry Graduate School Of Science Osaka University
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Yasuda Keiko
Graduate School Of Humanities And Sciences Nara Women's University:department Of Biology Nara W
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Oishi T
Graduate School Of Humanities And Sciences Nara Women's University:department Of Biological Sci
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Oishi Tadashi
Graduate School Of Human Culture Nara Women's University
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Kobayashi Kana
Faculty Of Letters Keio University
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Kobayashi K
Graduate School Of Humanities And Sciences Nara Women's University
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Kobayashi Kayo
Graduate School Of Humanities And Sciences Nara Women's University
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Tamotsu Satoshi
Graduate School Of Humanities & Sciences Nara Women's University
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Tamotsu Satoshi
Department Of Biological Science Nara Women's University
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Tamotsu Satoshi
First Department Of Physiology Hamamatsu University School Of Medicine
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