Release of Ecdysteroid-Phosphates from Egg Yolk Granules and Their Dephosphorylation during Early Embryonic Development in Silkworm, Bombyx mori(Endocrinology)

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概要

• 論文の詳細を見る

• Newly laid eggs of many insect species store maternal ecdysteroids as physiologically inactive phosphoric esters. In the silkworm Bombyx mori, we previously reported the presence of a specific enzyme, called ecdysteroid-phosphate phosphatase (EPPase), which catalyzes the dephosphorylation of ecdysteroid-phosphates to increase the amount of free ecdysteroids during early embryonic development. In this study, we demonstrated that (1) EPPase is found in the cytosol of yolk cells, (2) ecdysteroid-phosphates are localized in yolk granules, being bound to the yolk protein vitellin (Vn), and (3) Vn-bound ecdysteroid-phosphates are scarcely hydrolyzed by EPPase, although free ecdysteroid-phosphates are completely hydrolyzed by EPPase. Thus, we investigated the mechanism by which ecdysteroid-phosphates dissociate from the Vn-ecdysteroid-phosphate complex, and indicated that the acidification of yolk granules causes the dissociation of ecdysteroid-phosphates from the Vn-ecdysteroid-phosphate complex and thereby ecdysteroid-phosphates are released from yolk granules into the cytosol. Indeed, the presence of vacuolar-type proton-translocating ATPase in the membrane fraction of yolk granules was also verified by Western blot analysis. Our experiments revealed that Vn functions as a reservoir of maternal ovarian ecdysteroid-phosphates as well as a nutritional source during embryonic development. This is the first report showing the biochemical mechanism by which maternal Vn-bound ecdysteroid-phosphates function during early embryonic development.

• 社団法人日本動物学会の論文
• 2005-02-25

著者

• Sonobe Haruyuki

  Department of Biology, Faculty of Science and Engineering, Konan University

• Yamamoto Yoshimi

  Department Of Biology Hamamatsu University School Of Medicine

• Sonobe H

  Konan Univ. Kobe Jpn

• Sonobe Haruyuki

  Department Of Biophysical Chemistry Kobe Pharmaceutical University

• Yamahama Yumi

  Department of Biology, Hamamatsu University School of Medicine

• Yamahama Yumi

  Department Of Biology Hamamatsu University School Of Medicine

• Yamahama Yumi

  Department Of Biology Faculty Of Science Yamagata University:(present Address)department Of Biology

• Sonobe Haruyuki

  Department Of Biology Faculty Of Liberal Arts And Science Yamagata University

• Yamada Ryouichi

  Department of Life and Functional Material Science, Graduate School of Natural Sciences, Konan Unive

• Yamada R

  Department Of Life And Functional Material Science Graduate School Of Natural Sciences Konan Univers

• Yamada Ryouichi

  Department Of Biology Konan University

• SAITO Hiroyuki

  The University of Tokushima

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