Measurement of Molt-inhibiting Hormone Titer in Hemolymph of the American Crayfish, Procambarus clarkii, by Time-Resolved Fluoroimmunoassay(Endocrinology)
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概要
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In order to determine the titer of molt-inhibiting hormone (Prc-MIH) in the hemolymph of the American crayfish Procambarus clarkii, a time-resolved fluoroimmunoassay (TR-FIA) was established using specific antibodies against N-terminal and C-terminal segments of Prc-MIH. The lowest limit of detection of Prc-MIH in TR-FIA was 10 amol/assay. The Prc-MIH titers in the hemolymph were 6.53 fmol/ml at the intermolt stage and 1.25 fmol/ml at the early premolt stage. This result is consistent with the long-known hypothesis that the Y-organ is inhibited by MIH during the intermolt stage, whereas the Y-organ is activated by being freed from the inhibitory regulation of MIH.
- 社団法人日本動物学会の論文
- 2003-08-25
著者
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Sonobe Haruyuki
Department of Biology, Faculty of Science and Engineering, Konan University
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Sonobe Haruyuki
Department Of Life And Functional Material Science Graduate School Of Natural Sciences Konan Univers
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NAKATSUJI Teruaki
Department of Biology, Faculty of Science, Konan University
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Sonobe Haruyuki
Department Of Biology Faculty Of Liberal Arts And Science Yamagata University
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Nakatsuji T
Department Of Life And Functional Material Science Graduate School Of Natural Sciences Konan Univers
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